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MABE1035 Anti-ZBTB46 (ZNF340) Antibody, clone 5A8.1

Anti-ZBTB46 (ZNF340), clone 5A8.1 Antibody, Cat. No. MABE1035, is a highly specific mouse monoclonal antibody, that targets ZBTB46 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-ZBTB46 (ZNF340) Antibody, clone 5A8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ZBTB46, BTBD4, ZNF340 UniProt Number: Q86UZ6
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MABE1035
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1035
      DescriptionAnti-ZBTB46 (ZNF340) Antibody, clone 5A8.1
      Alternate Names
      • Zinc finger and BTB domain-containing protein 46
      • BTB-ZF protein expressed in effector lymphocytes
      • BTB/POZ domain-containing protein 4
      • BZEL
      • Zinc finger protein 340
      Background InformationZinc finger and BTB domain-containing protein 46 (UniProt Q86UZ6; also known as BTB-ZF protein expressed in effector lymphocytes, BTB/POZ domain-containing protein 4, BZEL, Classical dendritic cell-restricted zinc finger transcription factor zDC, Zinc finger protein 340) is encoded by the ZBTB46 (also known as BTBD4, ZNF340) gene (Gene ID 140685) in human. ZBTB46/ZNF340 is a broad complex, tramtrack, bric-a-brac and zinc finger (BTB-ZF) protein expressed in classical dendritic cells (cDCs), but not other immune cell populations, including plasmacytoid DCs, monocytes, or macrophages. Genome-wide ChIP-seq analysis reveals that ZBTB46/ZNF340 associates with more than 1,000 gene promoters and acts as a transcriptional repressor against many of these target genes. Zbtb46-knockout profoundly alters the cDC subset composition in the spleen in favor of CD8+ DCs, as well as upregulates activation pathways in steady-state cDCs, including elevated creb1 and MHC II expression, and enhances cDC production of vascular endothelial growth factor, leading to increased vascularization of skin-draining lymph nodes. Human ZBTB46/ZNF340 is a 589-a.a. protein containing a BTB domain (a.a. 31-99) and two C2H2-type Zinc fingers (a.a. 418-436, 446-468).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ZBTB46 (ZNF340), clone 5A8.1 Antibody, Cat. No. MABE1035, is a highly specific mouse monoclonal antibody, that targets ZBTB46 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected ZBTB46 (ZNF340) in human cererbral cortex, kidney, and tonsil tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant internal fragment from the N-terminal half of human ZBTB46 (ZNF340).
      Clone5A8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5A8.1 targets an internal epitope within the N-terminal half of human ZBTB46 (ZNF340).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ZBTB46
      • BTBD4
      • ZNF340
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~70 kDa observed. Target band size appears larger than the calculated molecular weight of 64.08 kDa. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human ovary tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZBTB46 (ZNF340) in 10 µg of human ovary tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1035 04054839057267