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16-210

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate

clone 2F3.2, Upstate®, from mouse

Synonym(s):

70 kDa zeta-associated protein, Syk-related tyrosine kinase, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
FITC conjugate
Clone:
2F3.2, monoclonal
Application:
FACS, ICC, WB
Citations:
7
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biological source

mouse

Quality Segment

100

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2F3.2, monoclonal

species reactivity

human, rat

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

isotype

IgG2a

NCBI accession no.

NP_001070

UniProt accession no.

P43403

shipped in

wet ice

target post-translational modification

unmodified

General description

70 kDa
Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals

Product Description: Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC)

Immunogen: GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to amino acids 1-254
ZAP70, a 70 kDa member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.

Immunogen

GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 correspon-ding to amino acids 1-254. Clone 2F3.2.

Application

Anti-ZAP-70 Antibody, clone 2F3.2, FITC conjugate detects level of ZAP-70 & has been published & validated for use in FC, IC & WB.
Immunocytochemistry:
5-20 µg/mL of a previous lot detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin.
Research Category
Signaling
Research Sub Category
Immunological Signaling

Biochem/physiol Actions

Expected to cross-react with rat.
Recognizes human ZAP-70.

Physical form

Mouse monoclonal FITC-conjugated IgG2a in buffer containing PBS containing 0.05% sodium azide.

Protein G purified mouse IgG2a, Catalog # 05-253, conjugated to fluorescein isothiocyanate (FITC). Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
Protein G Purified

Preparation Note

Stable for 6 months at 2-8°C from date of receipt. For maximum recovery of product, centrifuge original vial prior to removing cap.

Analysis Note

Control
Western Blot: Jurkat cell lysate.
Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis:
1:500 dilution of this lot detected ZAP 70 on 10 μg of Jurkat lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

16-210:

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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