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MABD136 Anti-Wnt-5a Antibody, clone 6F2

Detect Wnt-5a using this mouse monoclonal antibody, Anti-Wnt-5a Antibody, clone 6F2 validated for use in western blotting, Immunofluorescence & IHC.

Anti-Wnt-5a Antibody, clone 6F2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: WNT5A, Wnt-5a UniProt Number: P41221
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MABD136
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IF, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABD136
      DescriptionAnti-Wnt-5a Antibody, clone 6F2
      Alternate Names
      • Protein Wnt-5a
      Background InformationThe Wnt family of cysteine-rich proteins constitutes a group of ligands known for their roles in development such as cell differentiation, proliferation, migration and embryonic patterning. They have also been implicated in the formation of tumors. Wnts are involved in the stimulation of several signaling cascades that have been divided into two types; the canonical pathway, and the non-canonical pathway. Wnt-5a is considered to be a non-canonical ligand for the Wnt signaling pathway and is a ligand to the frizzled family of seven transmembrane receptors. When interacting with ROR2, Wnt-5a is considered to inhibit tumor formation due to Wnt-5a’s suppression of the canonical Wnt signaling pathway. Wnt-5a is involved in activating cell migration, regulating the motility of melanoma cells, decreasing the proliferation, chondrogenesis, and migration of carcinoma cells, and inhibiting the expression of type II collagen in chondrocytes. Wnt-5a is a requirement to the endothelial differentiation and vascularization of Embryonic Stem Cells, and is also necessary to the development of mammary glands, limb outgrowth, and the genital tubercle.
      References
      Product Information
      FormatAscites
      Control
      • Wnt-5a-hIgGFc transfected HEK293 cell lysate
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Wnt-5a using this mouse monoclonal antibody, Anti-Wnt-5a Antibody, clone 6F2 validated for use in western blotting, Immunofluorescence & IHC.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected Wnt-5a in human lung cancer, thyroid cancer, lymph node, and brain tissues.

      Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected Wnt-5a in PC-12 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human Wnt-5a expressed in E. Coli.
      Clone6F2
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • WNT5A
      • Wnt-5a
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~70 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Wnt-5a-hIgGFc transfected HEK293 cell lysate.

      Western Blotting Analysis: A 1:500-2000 dilution of this antibody detected Wnt-5a in Wnt-5a-hIgGFc transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABD136 04053252916465