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MABE1025 Anti-Tankyrase-2 Antibody, clone 2F5.1

Anti-Tankyrase-2 Antibody, clone 2F5.1 is an antibody against Tankyrase-2 for use in Western Blotting.

Anti-Tankyrase-2 Antibody, clone 2F5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: General Post-translation Modification Gene Symbol: TNKS2, PARP5B, TANK2, TNKL UniProt Number: Q9H2K2
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MABE1025
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1025
      DescriptionAnti-Tankyrase-2 Antibody, clone 2F5.1
      Alternate Names
      • TANK2
      • Tankyrase-2
      • ADP-ribosyltransferase diphtheria toxin-like 6
      • ARTD6
      • Poly [ADP-ribose] polymerase 5B
      • TNKS-2
      • TRF1-interacting ankyrin-related ADP-ribose polymerase 2
      • Tankyrase II
      • Tankyrase-like protein
      • Tankyrase-related protein
      Background InformationTankyrase-2 (EC 2.4.2.30; UniProt Q9H2K2; also known as ADP-ribosyltransferase diphtheria toxin-like 6, ARTD6, PARP-5B, Poly [ADP-ribose] polymerase 5B, TANK2, TNKS-2, Tankyrase II, Tankyrase-like protein, Tankyrase-related protein, TRF1-interacting ankyrin-related ADP-ribose polymerase 2) is encoded by the TNKS2 (also known as PARP5B, TANK2, TNKL) gene (Gene ID 80351) in human. Tankyrase-2 belongs to the poly(ADP-ribosylation) polymerase (PARP) family of enzymes that mediate post-translational modification of proteins by poly(ADP-ribosyl)ation, where repeating units of ADP-ribose linked by glycosidic bonds were added to aspartate, glutamate, asparagine, arginine or lysine residues of substrate proteins. Poly(ADP-ribose) (PAR) modification is reversible through the action of poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl protein lyase. Tankyrase-2 and the related Tankyrase-1 share a common domain organization with 15 N-terminal ankyrin-repeats (a.a. 57-776 in TNKS2) involved in substrate recognition, a sterile alpha motif (SAM) domain (a.a. 873-936 in TNKS2) required for dimerization, followed by the C-terminal PARP domain (a.a. 959-1164 in TNKS2). Mice with either Tnks1- or Tnks2-knockout are viable, while double Tnks1-/Tnks2-knockout is lethal at embryonic day 9.5, suggesting a functional redundancy between these two enzymes. Both tankyrases target and suppress Axin2, a well known β-catenin negative regulator. Tankyrase-2 ribosylates Src-binding adapter protein 3BP2, leading to its ubiquitination and proteasomal degradation in macrophages and osteoclasts. Loss of tankyrase-mediated 3BP2 negative regulation underlies the pathogenic mechanism of cherubism, an autosomal dominant disorder affecting cranial bone development.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tankyrase-2 Antibody, clone 2F5.1 is an antibody against Tankyrase-2 for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human Tankyrase-2 fragment from the region between ANK repeats 6 & 8.
      EpitopeRegion between ANK repeats 6 & 8.
      Clone2F5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2F5.1 recognizes an epitope in the region between ANK repeats 6 & 8 of human Tanyrase-2.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TNKS2
      • PARP5B
      • TANK2
      • TNKL
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~126 kDa observed. 126.9 kDa (calculated). Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Tankyrase-2 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1025 04055977350906