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MABC286 Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2

This Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of Tankyrase-1/TNKS.

Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: TNKS, PARP5A, PARPL, TIN1, TINF1, TNKS1 UniProt Number: O95271
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MABC286
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC286
      DescriptionAnti-Tankyrase-1/TNKS Antibody, clone 7D1.2
      Alternate Names
      • Tankyrase-1
      • TANK1
      • ADP-ribosyltransferase diphtheria toxin-like 5
      • ARTD5
      • Poly [ADP-ribose] polymerase 5A
      • TNKS-1
      • TRF1-interacting ankyrin-related ADP-ribose polymerase
      • Tankyrase I
      Background InformationTankyrase-1/TNKS is a critical enzyme in Wnt signaling pathway. Tankyrase-1/TNKS is a poly-ADP-ribosyltransferase which is a key mediator of PARsylation: Becoming ADP-ribosylated. PARsylation is important in Wnt signaling because ADP-ribosylation of AXIN1 and AXIN2, two key components of the beta catenin destruction complex, is necessary for destruction via RNF146 (an ubiquitin lyase) and ubiquitination and subsequent destruction of beta catenin. Tankyrase-1/TNKS also mediates PARsylation of TERF1 and thus is important in controlling telomere length, and Tankyrase-1/TNKS’s PARsylation of other proteins makes Tankyrase-1/TNKS important in vesicle trafficking and subcellular protein distribution as well. Tankyrase-1/TNKS is widely expressed, and is found mostly cytoplasmically but can be associated with the nucleus and spindles and Golgi complexes as well.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 is validated for use in Western Blotting and Immunocytochemistry and Immunohistochemistry for the detection of Tankyrase-1/TNKS.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Tankyrase-1/TNKS in Hela, A431, and N1H/3T3 cells.

      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Tankyrase-1/TNKS in neurons and glial cells in human cerebral cortex tissue, Purkinje, basket and granule cells in human cerebellum tissue, and neurons in human thalamus tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to Human Tankyrase-1/TNKS.
      Clone7D1.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG3κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TNKS
      • PARP5A
      • PARPL
      • TIN1
      • TINF1
      • TNKS1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~142 kDa observed.
      Uncharacterized bands may be observed in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 4 µg/mL of this antibody detected Tankyrase-1/TNKS in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC286 04055977073294

      Documentation

      Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tankyrase-1/TNKS Antibody, clone 7D1.2 Certificates of Analysis

      TitleLot Number
      Anti-Tankyrase-1/TNKS, clone 7D1.2 - Q2468770 Q2468770
      Anti-Tankyrase-1/TNKS, clone 7D1.2 - 2562020 2562020
      Anti-Tankyrase-1/TNKS, clone 7D1.2 - 3286427 3286427
      Anti-Tankyrase-1/TNKS, clone 7D1.2 - 3840337 3840337
      Anti-Tankyrase-1/TNKS, clone 7D1.2 -2792426 2792426
      Anti-Tankyrase-1/TNKS, clone 7D1.2 Monoclonal Antibody 2988126