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MAB4381C3

Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate

clone TRA-1-81, from mouse, CY3 conjugate

Synonym(s):

Podocalyxin, GCTM-2 antigen, Gp200, Podocalyxin-like protein 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
CY3 conjugate
Clone:
TRA-1-81, monoclonal
Application:
ICC
Citations:
5
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biological source

mouse

Quality Segment

100

conjugate

CY3 conjugate

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

TRA-1-81, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable

isotype

IgM

NCBI accession no.

NP_001018121.1

UniProt accession no.

O00592

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PODXL(5420)

General description

420/250 kDa calculated
TRA-1-81 is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). Both TRA-1-81 and TRA-1-60 recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope while TRA-1-81 reacts with an unknown epitope of the same molecule. TRA-1-81 is commonly used as marker of undifferentiated pluripotent human stem cells and is downregulated upon differentiation. TRA-1-81 has recently been identified as podocalyxin.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Evaluated by Immunocytochemistry in mouse embryonic stem cells (SCR012). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody did not detect TRA-1-81 in mouse embryonic stem cells (SCR012).
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-TRA-1-81 Antibody, clone TRA-1-81, Cy3 conjugate is validated for use in ICC for the detection of TRA-1-81.

Biochem/physiol Actions

This antibody recognizes human TRA-1-81.

Physical form

Purified mouse monoclonal IgM conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Preparation Note

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
H9 human embryonic stem cells
Evaluated by Immunocytochemistry in H9 human embryonic stem cells.
Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected TRA-1-81 in H9 human embryonic stem cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MAB4381C3:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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