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MABN1806 Anti-TPP1 Antibody, clone 2E12

Anti-TPP1, clone 2E12, Cat. No. MABN1806, is a highly specific mouse monoclonal antibody, that targets TPP1/Tripeptidyl-peptidase 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.

Anti-TPP1 Antibody, clone 2E12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: TPP1, CLN2, GIG1, UNQ267/PRO304 UniProt Number: O14773
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MABN1806
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1806
      DescriptionAnti-TPP1 Antibody, clone 2E12
      Alternate Names
      • Tripeptidyl-peptidase 1
      • Cell growth-inhibiting gene 1 protein
      • Lysosomal pepstatin-insensitive protease
      • LPIC
      • Tripeptidyl aminopeptidase
      Background InformationTripeptidyl-peptidase 1 (UniProt: O14773; also known as TPP1, Cell growth-inhibiting gene 1 protein, Lysosomal pepstatin-insensitive protease, LPIC, Tripeptidyl aminopeptidase) is encoded by the TPP1 (also known as CLN2, GIG1, UNQ267/PRO304) gene (Gene ID: 1200) in human. TPP1 is a lysosomal serine protease that acts as a non-specific peptidase and sequentially removes tripeptides from the free N termini of small polypeptides produced by lysosomal proteinases. TPP1 is expressed in several tissues, with tissues associated with peptide hormone production displaying higher level of expression. Human TPP I is synthesized as a zymogen with an apparent mass of 68 kDa, which is processed to a 48 kDa mature enzyme by removal of 179 amino acid prodomain. TPP I zymogen has five potential N-glycosylation sites at amino acid positions 210, 222, 286, 313, and 443. The N-linked glycans can add around 10 kDa to the molecular mass of TPP 1. Like many other lysosomal hydrolases, TPP I proenzyme is able to autoactivate in the acidic pH in vitro. Mutations in TPP1 gene are shown to be a causative factor in ceroid lipofuscinosis, neuronal, 2 (CLN2) a progressive neurodegenerative, lysosomal storage disease, characterized by intracellular accumulation of autofluorescent liposomal material and clinically manifested seizures, dementia, visual loss, and/or cerebral atrophy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TPP1, clone 2E12, Cat. No. MABN1806, is a highly specific mouse monoclonal antibody, that targets TPP1/Tripeptidyl-peptidase 1 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected TPP1 in human heart and human pancreas tissue.

      Western Blotting Analysis: A 1:50 dilution from a representative lot detected TPP1 in CHO cells transfected with TPP1 and TPP1 mutant (Courtesy of Adam Golabek, Ph.D., New York State Institute for Basic Research in Developmental Disabilities, New York, USA).

      Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected TPP1 in CHO cells expressing WT TPP1 (Courtesy of Adam Golabek, Ph.D., New York State Institute for Basic Research in Developmental Disabilities, New York, USA).
      Biological Information
      ImmunogenA recombinant fragment corresponding to amino acids 195-563 of human TPP1.
      Clone2E12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2E12 specifically immunostains tripeptidyl peptidase 1 in human placenta, heart, and pancreas.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TPP1
      • CLN2
      • GIG1
      • UNQ267/PRO304
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight61.25 kDa calculated (composed of 6 identical subunits)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human placenta tissue.

      Immunohistochemistry Analysis: A 1:5,000 dilution of this antibody detected TPP1 in human placenta tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1806 04054839168680