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MABS1289 Anti-T4 Lysozyme (T4L) Antibody, clone 6A6

Anti-T4 Lysozyme (T4L) Antibody, clone 6A6, Cat. No. MABS1289, is a highly specific mouse monoclonal antibody that targets bacteriophage T4 lysozyme (T4L), validated for use in Immunoprecipitation and Western Blotting for the detection of T4L and T4L-tagged fusion proteins.

Anti-T4 Lysozyme (T4L) Antibody, clone 6A6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Gene Symbol: Bacteriophage T4 gene E UniProt Number: P00720
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MABS1289
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1289
      DescriptionAnti-T4 Lysozyme (T4L) Antibody, clone 6A6
      Alternate Names
      • Bacteriophage T4 endolysin
      • Bacteriophage T4 lysis protein
      • Bacteriophage T4 lysozyme
      • Bacteriophage T4 muramidase
      Background InformationBacteriophage T4 endolysin (EC 3.2.1.17; UniProt P00720; also known as T4 lysis protein, T4 lysozyme, T4 muramidase, T4L) is encoded by the T4 E gene. T4 lysozyme (T4L) is a 164-a.a. monomeric protein that hydrolyzes peptidoglycan.T4L functions outside the cytoplasmic membrane and starts the phage extracellular phase by liberating virions from the metabolically inert remains of the bacterial host. Similar to glutathione S-transferase (GST) and T7 lysozyme, T4L sequence is commonly utilized to generate soluble recombinant fusions of hydrophobic proteins, such as multitransmembrane proteins, that are otherwise not soluble when expressed entirely in their native sequences. Antibodies against T4L are useful in detecting T4L in T4-infected bacteria hosts and in probing recombinant T4L fusion proteins.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-T4 Lysozyme (T4L) Antibody, clone 6A6, Cat. No. MABS1289, is a highly specific mouse monoclonal antibody that targets bacteriophage T4 lysozyme (T4L), validated for use in Immunoprecipitation and Western Blotting for the detection of T4L and T4L-tagged fusion proteins.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 1 µg from a representative lot immunoprecipitated exogenously expressed TL4 fusion protein from 1.5 µg of lysate of transfected cells by an independent laboratory.
      Biological Information
      ImmunogenRecombinant T4L fusion protein.
      Clone6A6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6A6 detects T4L and recombinant T4L fusion proteins.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteBacteriophage T4.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Bacteriophage T4 gene E
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight18.69 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blotting Analysis: 0.1 µg/mL of this antibody detected exogenously expressed T4L fusion protein in 0.2 µg of lysate from transfected cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1289 04054839057229