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MAC143-25UL Anti-Strep-Tag Antibody, clone C23.21

Anti-Strep-Tag, clone C23.21, Cat. No. MAC143, is a mouse monoclonal antibody that detects Strep-Tagged proteins and is tested for use in Immunohistochemistry, Surface plasmon resonance, and Western Blotting.

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MAC143-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAC143-25UL
      DescriptionAnti-Strep-Tag Antibody, clone C23.21
      Alternate Names
      • Strep-Tag II
      Background InformationStrep-Tag is a short amino acid-peptide sequence that exhibits specific binding to streptavidin. It is shown to reversibly occupy the same pocket where biotin is normally complexed. Hence, it is highly desirable for applications involving where efficient purification of fusion proteins. Use of this tag allows for easier detection of fusion proteins in various immunochemical assays. Strep-Tag is shown to be resistant to the action of proteases and can also be used in the presence of mild detergents. Clone C23.21 recognizes fusion proteins with Strep-Tag peptide sequence WSHPQFEK with picomolar affinity. It recognizes both the single and the double Strep-Tag sequence.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Strep-Tag, clone C23.21, Cat. No. MAC143, is a mouse monoclonal antibody that detects Strep-Tagged proteins and is tested for use in Immunohistochemistry, Surface plasmon resonance, and Western Blotting.
      Key Applications
      • Western Blotting
      • Surface plasmon resonance
      • Immunohistochemistry
      Application NotesSurface plasmon resonance: A representative lot detected Strep-Tag in Surface plasmon resonance application. (Meola, A., et al. (2015). J Virol. 89(4):2170-81).

      Western Blotting Analysis: A representative lot detected Strep-Tag in human brain extract and GFAP in Western Blotting applications. (Li, T., et al. (2017). Immunol Lett. 188:89-95).

      Immunohistochemistry Applications: A representative lot detected Strep-Tag in mouse brain tissue sections in Immunohistochemistry applications (Li, T., et al. (2017). Immunol Lett. 188:89-95).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant ectodomain of glycoprotein E2 of GB virus (GBV-B), containing a double Strep-Tag (two Strep-Tags separated by a linker peptide)
      EpitopeUnknown
      CloneC23.21
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone C23.21 is a mouse monoclonal antibody that detects Strep-Tagged proteins. It binds to WSHPQFEK sequence with picomolar affinity.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteAll.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with Strep-Tagged VHH 9.

      Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Strep-Tagged VHH9.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAC143-25UL 04065266876192