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MABT321 Anti-Septin-9 Antibody, clone 1A6C2

Anti-Septin-9 Antibody, clone 1A6C2 is an antibody against Septin-9 for use in Western Blotting and Immunocytochemistry.

Anti-Septin-9 Antibody, clone 1A6C2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: 41891 UniProt Number: Q9QZR6
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MABT321
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT321
      DescriptionAnti-Septin-9 Antibody, clone 1A6C2
      Alternate Names
      • Septin-9
      • Eighth septin
      • Eseptin
      • Septin-like protein
      • SLP
      Background InformationSeptin-9 (also known as eighth septin, Eseptin, and MLL septin-like fusion) is encoded by the Sept9 gene in rat and mouse (SEPT9 in human). Septins are GTP-binding proteins capable of forming higher-order oligomers and filamentous polymers that contact with the cell membrane and cytoskeleton. Septins are involved in various cellular processes, including cytokinesis, karyokinesis, exocytosis, and cell shape maintenance. The expression of SEPT2, SEPT6, SEPT7 and SEPT9 in heart tissue is reported to correlate well with cardiomyocyte cytokinesis activity during heart development, is highly expressed in embryonic heart, downregulated around birth, and nondetectable in the adult heart. (PMID 16739252 & 24837489). Clone 1A6C2 was generated using an immunogen sequence present only in rat spetin-9 isoform 1 (SLP-a; Q9QZR6), but missing in rat isoform 2-4 (SLP-b, E-septin long & short forms). The immunogen sequence is 100% conserved in murine species (A2A6U3) and differs by one amino acid when compared to the corresponding human sequence in isoform 2, and missing in human isoforms 1,3,4 (Q9UHD8).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Septin-9 Antibody, clone 1A6C2 is an antibody against Septin-9 for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Septin-9 in HeLa cells (Dr. Taro Tachibana, Cell Engineering Corporation).
      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Septin-9 in HeLa cell lysate (Dr. Taro Tachibana, Cell Engineering Corporation).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of rat Septin-9.
      EpitopeN-terminus
      Clone1A6C2
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • 41891
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~65 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected Septin-9 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT321 04055977161588