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05-1317 Anti-SUZ12 Antibody, clone 2AO9

Use Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein.

Anti-SUZ12 Antibody, clone 2AO9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: SUZ12, CHET9, JJAZ1 UniProt Number: Q15022
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05-1317
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RbIP, ICC, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1317
      DescriptionAnti-SUZ12 Antibody, clone 2AO9
      Alternate Names
      • ChET 9 protein
      • Chromatin precipitated E2F target 9 protein
      • Joined to JAZF1 protein
      • Suppressor of zeste 12 protein homolog
      • joined to JAZF1
      • suppressor of zeste 12 homolog (Drosophila)
      Background InformationSUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein.
      Key Applications
      • Immunoprecipitation
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.

      Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.
      Biological Information
      ImmunogenRecombinant protein corresponding to human SUZ12.
      EpitopeUnknown
      Clone2AO9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes SUZ12.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rabbit
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq]
      Gene Symbol
      • SUZ12
      • CHET9
      • JJAZ1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3.

      SIZE: 739 amino acids; 83055 Da

      SUBUNIT: Component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like BMI1, CBX4, CBX8, PHC2, PHC3, SCMH1, RING1 and RNF2.

      SUBCELLULAR LOCATION: Nucleus (Probable).

      DISEASE: chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors. Translocation t(7;17)(p15;q21) with JAZF1. The translocation generates the JAZF1-SUZ12 oncogene consisting of the N-terminus part of JAZF1 and the C-terminus part of SUZ12. It is frequently found in all cases of endometrial stromal tumors, except in endometrial stromal sarcomas, where it is rarer.

      SIMILARITY: Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. & Contains 1 C2H2-type zinc finger.
      Molecular Weight~ 95 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunoprecipitation in HeLa cell lysate.

      Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1317 04053252321986

      Documentation

      Anti-SUZ12 Antibody, clone 2AO9 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SUZ12 Antibody, clone 2AO9 Certificates of Analysis

      TitleLot Number
      Anti-SUZ12, clone 2AO9 - 2029644 2029644
      Anti-SUZ12, clone 2AO9 - 2224468 2224468
      Anti-SUZ12, clone 2AO9 - 2291706 2291706
      Anti-SUZ12, clone 2AO9 - 3386427 3386427
      Anti-SUZ12, clone 2AO9 - 3701836 3701836
      Anti-SUZ12, clone 2AO9 - NG1726980 NG1726980
      Anti-SUZ12, clone 2AO9 - NG1800879 NG1800879
      Anti-SUZ12, clone 2AO9 - NG1857502 NG1857502
      Anti-SUZ12, clone 2AO9 - NRG1665726 NRG1665726
      Anti-SUZ12, clone 2AO9 -2664247 2664247
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies