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MABS1139 Anti-SUMO2/3 Antibody, clone 3H12

Detect SUMO2/3 and SUMO2/3-modified (SUMOylated) proteins using this rat monoclonal Anti-SUMO2/3 Antibody, clone 3H12, Cat. No. MABS1139, validated for use in Immunocytochemistry and Western blotting.

Anti-SUMO2/3 Antibody, clone 3H12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SUMO2, SMT3B, SMT3H2, SUMO3, SMT3A, SMT3H1 UniProt Number: P61956, P55854
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MABS1139
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, ICCRUnpurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1139
      DescriptionAnti-SUMO2/3 Antibody, clone 3H12
      Alternate Names
      • Small ubiquitin-related modifier 2
      • HSMT3
      • Sentrin-2
      • SMT3 homolog 2
      • Smt3B
      • SUMO-2
      • Ubiquitin-like protein SMT3B
      • Small ubiquitin-related modifier 3
      • SMT3 homolog 1
      • Smt3A
      • SUMO-3
      • Ubiquitin-like protein SMT3A
      Background InformationSmall ubiquitin-related modifier 2 (UniProt P61956; a.k.a. HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; a.k.a. SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (a.k.a. SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (a.k.a. SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein posttranslation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). As is the case with ubiquitination, SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the deSUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares approximately 50% and SUMO4 shares about 87% identity with SUMO2/3. SUMO4 is the only SUMO member whose ctivation and conjugation has not been demonstrated. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator.
      References
      Product Information
      FormatUnpurified
      PresentationRat IgG2a hybridoma culture supernatant with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect SUMO2/3 and SUMO2/3-modified (SUMOylated) proteins using this rat monoclonal Anti-SUMO2/3 Antibody, clone 3H12, Cat. No. MABS1139, validated for use in Immunocytochemistry and Western blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: Undiluted hybridoma culture supernatant from a representative lot detected SUMO2/3 immunoreactivity in 4% paraformaldehyde-fixed, 0.3% Triton X-100-permeabilized HeLa cells.

      Immunocytochemistry Analysis: 10 µg/mL of purified clone 3H12 detected SUMO2/3 immunoreactivity in primary mouse neural progenitor cells (Courtesy of Taro Tachibana, Ph.D., Osaka City University, Japan).

      Western Blotting Analysis: 1 µg/mL of purified clone 3H12 detected SUMOylated proteins in HeLa cell lysate (Courtesy of Taro Tachibana, Ph.D., Osaka City University, Japan).

      Immunocytochemistry Analysis: A representative lot detected SUMO-2/3 nuclear immunoreactive foci co-localized with those of MCAF1 and SUMO1 and enriched in heterochromatin proteins by indirect immunofluorescent staining of exponentially growing C-33A cells fixed with 4% paraformaldehyde and permeabilized by 0.2% Triton X-100. Trasfecting C-33A cells with SUMO2/3 shRNA or Discosoma sp. red fluorescent protein (DsRed) fusion containg SUMO2/3-interacting sequence (MCAF1 a.a. 965-975) abolished nuclear staining by clone 3H12 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).
      Biological Information
      ImmunogenGST-tagged recombinant full-length human SUMO3 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).
      Clone3H12
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 3H12 detects SUMO2/3 and SUMO2/3-modified (SUMOylated) proteins. Clone 3H12 reacts with SUMO2/3, but not SUMO1 (Uchimura, Y., et al. (2006). J. Biol. Chem. 281(32):23180-23190).
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with a broad-range of mammalian species based on high sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SUMO2
      • SMT3B
      • SMT3H2
      • SUMO3
      • SMT3A
      • SMT3H1
      Purification MethodUnpurified.
      UniProt Number
      Molecular WeightVariable depending on the degree of SUMOylation and the size(s) of the SUMOylated protein(s). 10.61/10.52 kDa (SUMO2/SUMO3; propeptide removed) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this hybridoma culture supernatant detected SUMO2/3-modified (SUMOylated) proteins in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1139 04054839132704