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ABS1640 Anti-SPTLC3 Antibody

Detect SPTLC3 using this rabbit polyclonal Anti-SPTLC3 Antibody, Cat. No. ABS1640, validated for use in Western Blotting.

Anti-SPTLC3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SPTLC3, C20orf38, SPTLC2L UniProt Number: Q9NUV7
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ABS1640
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbUnpurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1640
      DescriptionAnti-SPTLC3 Antibody
      Alternate Names
      • Serine palmitoyltransferase 3
      • LCB 3
      • LCB2b
      • Long chain base biosynthesis protein 2b
      • Long chain base biosynthesis protein 3
      • Serine-palmitoyl-CoA transferase 3
      • SPT 3
      Background InformationSerine palmitoyltransferase 3 (EC 2.3.1.50; UniProt Q9NUV7; also known as LCB 3, LCB2b, Long chain base biosynthesis protein 2b, Long chain base biosynthesis protein 3, Serine-palmitoyl-CoA transferase 3, SPT 3) is encoded by the SPTLC3 (also known as C20orf38, SPTLC2L) gene (Gene ID 55304) in human. Serine palmitoyltransferase (SPT) is a pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyses the first step of de novo sphingolipids (SLs) biosynthesis, namely the condensation of L-serine with palmitoyl coenzyme-A to generate 3-ketodehydrosphinganine (KDS) that forms the sphingoid base backbone of all SLs. The core human SPT homoenzyme is a membrane-bound heterodimer composed of an hLCB1 (SPTLC1) subunit together with either an hLCB2a (SPTLC2) or an hLCB2b (SPTLC3) subunit. The hLCB1/hLCB2 heterodimer contains a single active site composed of residues from both subunits. hLCB2a and hLCB2b share 68% sequence identity, with hLCB2b being the isotype that is predominantly expressed in the placenta and prefers shorter chain acyl-CoAs (C12 and C14) to generate short-chain SLs. In addition, two human small subunits (ssSPTa and ssSPTb) have been identified and found to maximize the catalytic activity and to confer the acyl-CoA substrate chain-length specificity of the hLCB1/hLCB2 core enzyme. Mutations in hLCB1 and hLCB2a are found in patients with hereditary sensory and autonomic neuropathy type I (HSAN1), an inherited disorder that affects sensory and autonomic neurons due to neurotoxic deoxysphingolipids formation as a result of substrate promiscuity of the mutated SPT enzyme.
      References
      Product Information
      FormatUnpurified
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect SPTLC3 using this rabbit polyclonal Anti-SPTLC3 Antibody, Cat. No. ABS1640, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: The purified polyclonal antibody was used to detect the presence of SPTLC2 in SPTLC1 and SPTLC2 immunoprecipiates from human placenta tissue homogenate (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164).

      Western Blotting Analysis: The purified polyclonal antibody was used to detect the endogenous SPTLC3 in human cell lysates (HepG2, JAR, JEG3) and human placenta tissue homogenate, as well as exogenously expressed SPTLC3 in transfected HEK293 cells (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164; Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281).
      Biological Information
      ImmunogenA mixture of two linear peptides corresponding to sequences near the N- and C-terminal ends of human SPTLC3.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityTarget-specificity of this antiserum was verified by negative blotting using pre-immune serum and by target band blocking using immunogen peptides (Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281). Expected to react with both spliced isoforms of human Serine palmitoyltransferase 3/SPTLC3 reported by UniProt (Q9NUV7).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Non-Human Primate based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SPTLC3
      • C20orf38
      • SPTLC2L
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~52 kDa observed. 62.05 kDa (isoform 1) and 19.63 kDa (isoform 2) calculated. The smaller-than-calculated band size is consistent with that reported in the literature (Hornemann, T., et al. (2007). Biochem. J. 405(1):157-164; Hornemann, T., et al. (2006). J. Biol. Chem. 281(49):37275-37281). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human placenta tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SPTLC3 in 10 µg of human placenta tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1640 04054839049729