Cleavage of roquin and regnase-1 by the paracaspase MALT1 releases their cooperatively repressed targets to promote TH17 differentiation.
Jeltsch, KM; Hu, D; Brenner, S; Zöller, J; Heinz, GA; Nagel, D; Vogel, KU; Rehage, N; Warth, SC; Edelmann, SL; Gloury, R; Martin, N; Lohs, C; Lech, M; Stehklein, JE; Geerlof, A; Kremmer, E; Weber, A; Anders, HJ; Schmitz, I; Schmidt-Supprian, M; Fu, M; Holtmann, H; Krappmann, D; Ruland, J; Kallies, A; Heikenwalder, M; Heissmeyer, V
Nature immunology
15
1079-89
2014
Show Abstract
Humoral autoimmunity paralleled by the accumulation of follicular helper T cells (TFH cells) is linked to mutation of the gene encoding the RNA-binding protein roquin-1. Here we found that T cells lacking roquin caused pathology in the lung and accumulated as cells of the TH17 subset of helper T cells in the lungs. Roquin inhibited TH17 cell differentiation and acted together with the endoribonuclease regnase-1 to repress target mRNA encoding the TH17 cell-promoting factors IL-6, ICOS, c-Rel, IRF4, IκBNS and IκBζ. This cooperation required binding of RNA by roquin and the nuclease activity of regnase-1. Upon recognition of antigen by the T cell antigen receptor (TCR), roquin and regnase-1 proteins were cleaved by the paracaspase MALT1. Thus, this pathway acts as a 'rheostat' by translating TCR signal strength via graded inactivation of post-transcriptional repressors and differential derepression of targets to enhance TH17 differentiation. | 25282160
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Roquin paralogs 1 and 2 redundantly repress the Icos and Ox40 costimulator mRNAs and control follicular helper T cell differentiation.
Vogel, KU; Edelmann, SL; Jeltsch, KM; Bertossi, A; Heger, K; Heinz, GA; Zöller, J; Warth, SC; Hoefig, KP; Lohs, C; Neff, F; Kremmer, E; Schick, J; Repsilber, D; Geerlof, A; Blum, H; Wurst, W; Heikenwälder, M; Schmidt-Supprian, M; Heissmeyer, V
Immunity
38
655-68
2013
Show Abstract
The Roquin-1 protein binds to messenger RNAs (mRNAs) and regulates gene expression posttranscriptionally. A single point mutation in Roquin-1, but not gene ablation, increases follicular helper T (Tfh) cell numbers and causes lupus-like autoimmune disease in mice. In T cells, we did not identify a unique role for the much lower expressed paralog Roquin-2. However, combined ablation of both genes induced accumulation of T cells with an effector and follicular helper phenotype. We showed that Roquin-1 and Roquin-2 proteins redundantly repressed the mRNA of inducible costimulator (Icos) and identified the Ox40 costimulatory receptor as another shared mRNA target. Combined acute deletion increased Ox40 signaling, as well as Irf4 expression, and imposed Tfh differentiation on CD4(+) T cells. These data imply that both proteins maintain tolerance by preventing inappropriate T cell activation and Tfh cell differentiation, and that Roquin-2 compensates in the absence of Roquin-1, but not in the presence of its mutated form. | 23583643
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