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ABN1686 Anti-Rootletin

Anti-Rootletin Antibody, Cat. No. ABN1686, is a highly specific chicken polyclonal antibody that targets Rootletin and has been tested in Western Blotting.

Anti-Rootletin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Crocc, Kiaa0445 UniProt Number: Q8CJ40
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ABN1686
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, PorcineWBChAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1686
      DescriptionAnti-Rootletin
      Alternate Names
      • Ciliary rootlet coiled-coil protein
      Background InformationRootletin (UniProt: Q8CJ40; also known as Ciliary rootlet coiled-coil protein) is encoded by the Crocc (also known as Kiaa0445) gene (Gene ID: 230872) in murine species. Rootletin is a major structural component of the ciliary rootlet, a cytoskeletal-like structure in ciliated cells that originates from the basal body at the proximal end of a cilium and extends proximally toward the cell nucleus. It contributes to centrosome cohesion before mitosis. In ciliated cells, it is associated with ciliary rootlets, but in non-ciliated cells it is localized between, around and at the proximal ends of the centrioles. It dissociates from the centrioles at the onset of mitosis and re-associates with them at anaphase. Highest expression of Rootletin is found in photoreceptor cells of retina. Lower expressions have been reported in brain, trachea and kidney. In retinal photoreceptors rootlets extend from the basal bodies to the synaptic terminals and anchor endoplasmic reticulum membranes along their length. During embryonic development, it is enriched along the apical domains of neuro epithelium in brain ventricular zone, in primordia of retinal pigment epithelia, and in neural retina. Rootletin contains a globular head domain and a tail domain that consists of extended coiled-coil structures. The head domain may be required for targeting to the basal body and binding to a kinesin light chain. (Ref.: Yang, J., et al. (2002). J. Cell Biol. 159(3): 431-440).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified chicken polyclonal antibody in PBS with 50% glycerol and without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Rootletin Antibody, Cat. No. ABN1686, is a highly specific chicken polyclonal antibody that targets Rootletin and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Rootletin in Western Blotting applications (Yang, J., et. al. (2002). J Cell Biol. 159(3):431-40).

      Immunohistochemistry Analysis: A representative lot detected Rootletin in Immunohistochemistry applications (Yang, J., et. al. (2002). J Cell Biol. 159(3):431-40; Sun, X., etl al. (2016). Proc Natl Acad Sci USA. 113(21):E2925-34; Yadav, S.P., et. al. (2016). Development. 143(9):1491-501; Pawlyk, B.S., et. al. (2016). Gene Ther. 23(2):196-204; Sun X., et. al. (2012). Cilia. 1(1):21; Liu, X., et. al. (2007). Proc Antl Acad Sci USA. 104(11):4413-8; Bahe, S., et. al. (2005). J Cell Biol. 171(1):27-33; Yang, J., et. al. (2005). Mol Cell Biol. 25(10):4129-37; Hong, D.H, et. al. (2003). Invest Ophthalmol Vis Sci. 44(6):2413-21).
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 188 amino acids from the C-terminal region of murine Rootletin.
      ConcentrationPlease refer to lot specific datasheet.
      HostChicken
      SpecificityThis chicken polyclonal antibody detects Rootletin in mouse retina. It targets an epitope within 188 amino acids from the C-terminal region.
      IsotypeIgY
      Species Reactivity
      • Mouse
      • Human
      • Porcine
      Species Reactivity NoteMouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Crocc
      • Kiaa0445
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~227 kDa observed; 226.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse retina tissue lysate.

      Western Blotting Analysis: A 1,000 dilution of this antibody detected Rootletin in 10 µg of mouse retina tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1686 04054839193514