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ABC1391-25UG Anti-Ring Finger Protein 213 (RNF213)

Anti-Ring Finger Protein 213 (RNF213), Cat. No. ABC1391, is a rabbit polyclonal antibody that detects E3 ubiquitin-protein ligase RNF213 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-Ring Finger Protein 213 (RNF213) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: RNF213, ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR UniProt Number: Q63HN8
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ABC1391-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1391-25UG
      DescriptionAnti-Ring Finger Protein 213 (RNF213)
      Alternate Names
      • E3 ubiquitin-protein ligase RNF213
      • ALK lymphoma oligomerization partner on chromosome 17
      • Mysterin
      • RING finger protein 213
      • RING-type E3 ubiquitin transferase RNF213
      Background InformationE3 ubiquitin-protein ligase RNF213 (UniProt: Q63HN8; also known as ALK lymphoma oligomerization partner on chromosome 17, Mysterin, RING finger protein 213, RING-type E3 ubiquitin transferase RNF21) is encoded by the RNF213 (also known as ALO17, C17orf27, KIAA1554, KIAA1618, MYSTR) gene (Gene ID: 57674) in human. RNF213 is a widely expressed, homooligomeric, cytoplasmic protein that has E3 ubiquitin-protein ligase and AAA+ ATPase activity. It contains a zinc-finger domain (aa 3997-4036) that is required for its ubiquitin-protein ligase activity. RNF213 is shown to be involved in angiogenesis and in non-canonical Wnt signaling pathway in vascular development. Four isoforms of RNF213 have been described that are produced by alternative splicing. RNF213 ligase activity is negatively regulated by PTP1B in HER2+ breast cancer cells and RNF213 knockdown is shown to reverse the effects of PTP1B deficiency on alpha-keto-dependent dehydrogenases, non-mitochondrial oxygen consumption, and hypoxia-induced death of HER2+ BC cells. RNF213 activity is down-regulated by let-7c miRNA, which binds to the 3'-UTR transcript of RNF213. Microbial infection leading to induction of pro-inflammatory cytokines are shown to up-regulate its activity. Defects in RNF213 gene are known to cause Moyamoya disease 2, a progressive cerebral angiopathy characterized by bilateral intracranial carotid artery stenosis and telangiectatic vessels in the region of the basal ganglia, which can lead to transient ischemia and/or rupture of the collateral vessel. (Ref.: Banh, RS et al (2016). Nat. Cell Biol. 18(7); 803-813).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Ring Finger Protein 213 (RNF213), Cat. No. ABC1391, is a rabbit polyclonal antibody that detects E3 ubiquitin-protein ligase RNF213 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected Ring Finger Protein 213 (RNF213) in BT474, MDA-MB-361, and HCC1954 cells (Banh, R.S., et. al. (2016). Nat Cell Biol. 18(7):803-13).
      Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected Ring Finger Protein 213 (RNF213) in HUVEC transfected with siRNA213 (C ourtesy of Dr Akio Koizumi at Kyoto University) .
      Immunocytochemistry Analysis: A representative lot detected Ring Finger Protein 213 (RNF213) in HeLa cells treated with RNF213 siRNA (Hitomi, T., et. al. (2013). Biochem Biophys Res Commun. 438(1):13-9).
      Western Blotting Analysis: A representative lot detected Ring Finger Protein 213 (RNF213) in Western Blotting applications (Hitomi, T., et. al. (2013). Biochem Biophys Res Commun. 438(1):13-9; Banh, R.S., et. al. (2016). Nat Cell Biol. 18(7):803-13; Kobayashi, H., et. al. (2015). J Am Heart Assoc. 4(7)).
      Biological Information
      Immunogen11 GST-tagged recombinant fragments from human Ring Finger Protein 213.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human and mouse Ring Finger Protein 213.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • RNF213
      • ALO17
      • C17orf27
      • KIAA1554
      • KIAA1618
      • MYSTR
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~596 kDa observed; 591.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cells transfected with 3XFlag RNF213 wild-type.
      Western Blotting Analysis: 1 µg/mL of this antibody detected Ring Finger Protein 213 (RNF213) in HEK293T cells transfected with 3XFlag RNF213 wild-type.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1391-25UG 04054839518621