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MABT1586-25UG Anti-Profilin 1 Antibody, clone 2C5

Anti-Profilin 1, clone 2C5, Cat. No. MABT1586, is a mouse monoclonal antibody that detects Profilin 1 and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

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MABT1586-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1586-25UG
      DescriptionAnti-Profilin 1 Antibody, clone 2C5
      Alternate Names
      • Profilin-1
      Background InformationProfilin-1 (UniProt: P62962; also known as Profilin I) is encoded by the Pfn1 gene (Gene ID: 18643) in murine species. Five profilin isoforms have been identified in mammals: Profilins I, IIa, IIb, III and IV. They regulate actin dynamics at plasma membranes during the assembly, maintain and disassemble actin networks at the leading edge of locomoting animal cells during cytokinesis, and are involved in embryonic development and morphogenesis. Profilins bind to G-actin in a 1:1 complex and thereby sequesters monomeric actin. Profilin I is highly expressed in all tissues. It binds to actin and affects the structure of the cytoskeleton. In neurons, proper profilin 1 function is essential during development and is implicated in the formation and maintenance of the neuronal cytoskeleton, growth-cone formation, synaptogenesis, synaptic activities, shape and morphological dynamics, and the growth of dendrites and axons. Knockdown of both profilins I and II is reported to inhibit neurite outgrowth, possibly impairing actin dynamics by reducing the availability of profilin-G-actin complexes for microfilament growth. Higher levels of profilin 1 have been reported in subjects with severe aplastic anemia. G118V and C71G mutations in profilin 1 have been linked to Amyotrophic lateral sclerosis (ALS). (Ref.: Yu, H., et al. (2021). Front. Immunol. 12; 631954; Kaiei, M., et al. (2018). Sci. Rep. 8; Article 13102; Murk, K., et al. (2012). PLoS One. 7(3); e34167; Jockusch, BM., et al. (2007). Rev. Physiol. Biochem. Pharmacol. 159; 131-149).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Profilin 1, clone 2C5, Cat. No. MABT1586, is a mouse monoclonal antibody that detects Profilin 1 and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesTested Applications

      Immunocytochemistry Analysis: A representative lot detected Profilin-1 in Immunocytochemistry applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

      Western Blotting Analysis: A representative lot detected Profilin-1 in Western Blotting applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

      Immunofluorescence Analysis: A representative lot detected Profilin-1 in Immunofluorescence applications (Murk, K., et al. (2012). PLoS One. 7(3); e34167).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenA linear peptide corresponding to 14 amino acids from the N-terminal region of mouse Profilin-1.
      EpitopeN-terminal
      Clone2C5
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2C5 is a mouse monoclonal antibody that detects Profilin 1. It targets an epitope within 14 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pfn1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~15 kDa observed; 14.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Mouse spleen tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Profilin 1 in Mouse spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1586-25UG 04065268529171