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ABN1699 Anti-Prickle1

Anti-Prickle1 , Cat. No. ABN1699, is a highly specific rabbit polyclonal antibody that targets Prickle-like protein 1 and has been tested in Western Blotting.

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ABN1699
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABN1699
      DescriptionAnti-Prickle1
      Background InformationPrickle-like protein 1(UniProt: Q3U5C7) is encoded by the Prickle1 (also known as Pk1) gene (Gene ID: 106042) in murine species. Prickle Prickle-like Protein 1 is involved in the planar cell polarity (PCP) pathway that controls convergent extension during gastrulation and neural tube closure. PCP pathway comprises of multiple integral membrane proteins ((Frizzled, Flamingo/Stan/Celsr and Vangl/ Strabismus) and membrane-associated proteins (Dishevelled, Inversin, and Prickle). Prickle-like protein 1 is synthesized with a propeptide (aa 830-832) that is removed in mature protein. Prickle1 is expressed in distinct cell populations of the central nervous system and is a contributor to neuronal morphogenesis. Both Pk1 and Pk2 genes share three LIM (Lin 11, Isl-1 and Mec-3) and one PET domain (Prickle, Espinas and Testin) at the N-terminus of the protein and a CAAX prenylation motif at the C-terminus. Pk1 is shown to be expressed in the cortical subplate and is considered as an important site for early neuronal maturation. In the retinal tissue Pk1 expression is specific to neurons and is highly enriched in Starburst amarcine cells. Loss of Pk1 gene has been linked to early embryonic lethality.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Prickle1 , Cat. No. ABN1699, is a highly specific rabbit polyclonal antibody that targets Prickle-like protein 1 and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Prickle1 in Western Blotting applications (Liu, C., et. al. (2014). Biol Open. 3(9):861-70).
      Biological Information
      ImmunogenHis-tagged Recombinant Protein corresponding to the C-terminus of Mouse Prickle-like protein 1.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Prickle-like protein 1 in murine species.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Prickle1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~94 kDa observed; 94.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse retinal tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Prickle1 in mouse retinal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1699 04054839330667

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