MAB8566
Anti-Poliovirus Blend Antibody, clones 583-G8-G2-A4, 591-B1-H7-D1, and 613-F1-B5-E5
ascites fluid, Chemicon®, from mouse
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About This Item
biological source
mouse
Quality Segment
antibody form
ascites fluid
antibody product type
primary antibodies
clone
583-G8-G2-A4, monoclonal, 591-B1-H7-D1, monoclonal, 613-F1-B5-E5, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunofluorescence: suitable
isotype
IgG
shipped in
dry ice
Application
Dilute with buffer pH 7.5-8.0 to desired working volume.
Final working dilutions must be determined by end user.
SUGGESTED IFA PROTOCOL:
Substrate slides:
1. Scrape and spot cells onto microscope slide wells.
2. Air dry.
3. Fix in cold acetone for 10 minutes at +4°C.
Stain:
1. Add 15 μl diluted polio antibody.
2. Incubate at +37°C for 30 minutes in a humid chamber.
3. Rinse in PBS for 5 minutes; repeat with fresh PBS.
4. Add 15 μl diluted labeled antibody.
5. Repeat steps 2 and 3.
6. Mount with buffered glycerol.
Biochem/physiol Actions
Preparation Note
Legal Information
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
MAB8566:
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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