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MABN721 Anti-PTK7 Antibody, clone 4F9

Detect Protein-tyrosine kinase 7 using this mouse monoclonal antibody, Anti-PTK7 Antibody, clone 4F9 validated for use in western blotting & IHC.

Anti-PTK7 Antibody, clone 4F9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: CCK4, PTK7 UniProt Number: Q13308
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MABN721
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN721
      DescriptionAnti-PTK7 Antibody, clone 4F9
      Alternate Names
      • Inactive tyrosine-protein kinase 7
      • CCK-4
      • Protein-tyrosine kinase 7
      • Pseudo tyrosine kinase receptor 7
      • Tyrosine-protein kinase-like 7
      Background InformationThe gene PTK7 encodes the protein named Inactive tyrosine-protein kinase 7 or Protein-tyrosine kinase 7, or colon carcinoma kinase 4 (CCK4). The PTK7 encoded protein is an interesting single pass type 1 membrane protein involved in Wnt signaling. It is interesting because it contains an inactive tyrosine kinase domain yet it is a component of both the canonical Wnt and non-canonical signaling pathways, and operates during embryogenesis and angiogenesis. Moreover, the PTK7 protein functions in cell migration, cell adhesion, cell polarity, cytoskeleton reorganization and apoptosis, and the PTK7 encoded protein co-localizes with MMP14 at cell junctions as well as the leading edge of migrating cells. The PTK7 encoded protein is expressed in lung, liver, pancreas and other tissues like kidney, placenta and melanocytes. The PTK7 encoded protein does not seem to be expressed in colon and has low expression in brain, heart, and skeletal muscle. EMD-Millipore’s Anti-PTK7 mouse monoclonal, clone 4F9 has been tested by western blot against multiple human cancer cells lines and in immunohistochemistry using paraffin-embedded human lung cancer tissue with DAB detection.
      References
      Product Information
      FormatAscites
      Control
      • A431 cell lysate
      PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Protein-tyrosine kinase 7 using this mouse monoclonal antibody, Anti-PTK7 Antibody, clone 4F9 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100 and 1:500 dilution from a representative lot detected PTK7 in human lung and kidney tissues.
      Biological Information
      ImmunogenPurified protein fragment of human PTK7 expressed in E. coli.
      Clone4F9
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CCK4
      • PTK7
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~130 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 6 isoforms between ~90 kDa and ~119 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis:A 1:1,000 dilution of this antibody detected PTK7 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN721 04053252910272

      Documentation

      Anti-PTK7 Antibody, clone 4F9 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PTK7 Antibody, clone 4F9 Certificates of Analysis

      TitleLot Number
      Anti-PTK7, clone 4F9 - VP1403124 VP1403124
      Anti-PTK7, clone 4F9 - QVP1304250 QVP1304250
      Anti-PTK7, clone 4F9 - VP1407301 VP1407301
      Anti-PTK7, clone 4F9 - VP2106030 VP2106030
      Anti-PTK7, clone 4F9 - VP2203311 VP2203311
      Anti-PTK7, clone 4F9 - VP2205122 VP2205122
      Anti-PTK7, clone 4F9 - VP2206293 VP2206293
      Anti-PTK7, clone 4F9 - VP2403267 VP2403267
      Anti-PTK7, clone 4F9 -VP1501026 VP1501026
      Anti-PTK7, clone 4F9 -VP1508055 VP1508055
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