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MABC290

Anti-PDL-1/CD274 Antibody, clone 3F2.1

clone 3F2.1, 1 mg/mL, from mouse

Synonym(s):

Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, PDL-1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3F2.1, monoclonal
Application:
IHC, WB
Citations:
2
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biological source

mouse

Quality Segment

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F2.1, monoclonal

species reactivity

human

concentration

1 mg/mL

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001254635

UniProt accession no.

Q9NZQ7

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD274(29126)

General description

Programmed cell death 1 ligand 1 (PDL-1) is a B7 related protein belonging to the BTN/MOG family. PDL-1 is essential for T-cell proliferation and production of IL10 and IFNG. PDL-1 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PDL-1 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PDL-1 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.
~40 kDa observed. The calculated molecular weight is 33 kDa; however, this protein may be observed at higher molecular weights to due to heavy glycosylation.

Immunogen

Epitope: extracellular domain
GST-tagged recombinant protein corresponding to the extracellular domain of human PDL-1/CD274.

Application

Anti-PDL-1/CD274 Antibody, clone 3F2.1 is an antibody against PDL-1/CD274 for use in western blotting & IHC.
Immunohistochemistry Analysis: A 1:50 and 1:500 dilution from a representative lot detected PDL-1/CD274 in human tonsil and skeletal muscle tissue, respectively.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in human skeletal muscle tissue.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDL-1/CD274 in 10 µg of human skeletal muscle tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

MABC290:

jan

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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