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05-1135 Anti-PDGFR Antibody, clone CH-3

This Anti-PDGFR Antibody, clone CH-3 is validated for use in WB, ELISA, IH(P) for the detection of PDGFR.

Anti-PDGFR Antibody, clone CH-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling, Apoptosis & Cancer Research Sub-Category: Growth Factors & Receptors UniProt Number: P09619
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05-1135
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ELISA, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1135
      Replaces04-397
      DescriptionAnti-PDGFR Antibody, clone CH-3
      Alternate Names
      • Platelet Derived Growth Factor Receptor Beta
      • PDGF-R-beta
      • PDGFR-beta
      • Beta platelet-derived growth factor receptor
      • Beta-type platelet-derived growth factor receptor
      • CD140 antigen-like family member B
      • Platelet-derived growth factor receptor 1
      • PDGFR-1
      • CD140b
      Background InformationPDGFR (Platelet-Derived Growth Factor Receptor) is a receptor tyrosine kinase for the PDGF family of growth factors. PDGF homo and hetero- dimmers (dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB)) bind to the two structurally related a and b PDGF Receptors (PDGFRa and PDGFRb). The type A PDGF Receptor binds all three PDGFs with high affinity, whereas the type B PDGF Receptor is selective for PDGF-BB. This binding results in the dimerization of the receptor and the subsequent kinase activation of the receptor tyrosine kinase (RTK) and its autophosphorylation. These phosphorylations allow for various proteins, such as PI3 Kinase, PLCg, and Grb2, to bind through their SH2 domains and transduce signals into the cell. The activation of PDGFFR triggers the intracellular loop and activates Ras, Erk1/2, and ROS. PDGFb has been implicated in embryonic development, angiogenesis, and development of tumors of glial origin.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 20 mM sodium phosphate, 250 mM NaCl, 0.1% NaN3, pH. 7.6
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-PDGFR Antibody, clone CH-3 is validated for use in WB, ELISA, IH(P) for the detection of PDGFR.
      Key Applications
      • Western Blotting
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesELISA: A previous lot of this antibody was used in ELISA.

      Immunohistochemistry(paraffin): Representative images from a previous lot.
      Optimal Staining of PDGF-A With Citrate pH 6.0 Epitope Retrieval: Rat Brain
      Biological Information
      ImmunogenRecombinant Platelet-Derived Growth Factor Receptor
      CloneCH-3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse. Expected to react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight211 amino acids; 24043 Da
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Jurkat lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected PDGFR on 10 μg of Jurkat lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at + 2-8 ° C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-1135 04053252732973

      Documentation

      Anti-PDGFR Antibody, clone CH-3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PDGFR Antibody, clone CH-3 Certificates of Analysis

      TitleLot Number
      Anti-PDGFR-b, clone CH-3 (mouse monoclonal) Monoclonal Antibody Q2925488
      Anti-PDGFR-b, clone CH-3 - 2262721 2262721
      Anti-PDGFR-b, clone CH-3 - 2318054 2318054
      Anti-PDGFR-b, clone CH-3 - NG1572163 NG1572163
      Anti-PDGFR-b, clone CH-3 - NG1633532 NG1633532
      Anti-PDGFR-ß, clone CH-3 -2888561 2888561
      Anti-PDGFR-β, clone CH-3 - 2429681 2429681
      Anti-PDGFR-β, clone CH-3 - 3186892 3186892
      Anti-PDGFR-β, clone CH-3 - 4182462 4182462
      Anti-PDGFR-β, clone CH-3 -2582453 2582453
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