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MABN2301-100UG Anti-NLGN4X Antibody, clone CSTEM30

Anti-NLGN4X, clone CSTEM30, Cat. No. MABN2301, is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X) and is tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.

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MABN2301-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2301-100UG
      DescriptionAnti-NLGN4X Antibody, clone CSTEM30
      Alternate Names
      • Neuroligin-4 X-linked
      • Neuroligin X
      • HNLX
      Background InformationNeuroligin-4, X-linked (UniProt: Q8N0W4; also known as Neuroligin X, HNLX) is encoded by the NLGN4X (also known as KIAA1260, NLGN4, UNQ365/PRO701) gene (Gene ID: 57502) in human. NLGN4X is a single-pass type I membrane, homodimeric, glycoprotein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 42-676) a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). It is predominantly expressed in the heart tissue. It is also expressed at lower levels in the liver, skeletal muscle, pancreas, and brain. It is a putative neuronal cell surface protein that is involved in cell-cell-interactions. In human, NLGN4X is localized at excitatory synapses, whereas in mouse NLGN4-like is localized at glycinergic synapses. NLGN4X can be phosphorylated by protein kinase C that enhances excitatory synapses. Mutations in NLGN4X gene have been linked to X-linked Asperger syndrome and Autism. (Ref.: Nguyen, TA., et al. (2020). Front. Synaptic Neurosci. 12; 33; Laumonnier, F., et al. (2004). Am. J. Hum. Genet. 74(3); 552-557).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody Ig G2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-NLGN4X, clone CSTEM30, Cat. No. MABN2301, is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X) and is tested for use in ELISA, Flow Cytometry, and Immunocytochemistry.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • ELISA
      Application NotesTested applications

      Flow Cytometry Analysis: A representative lot detected NLGN4X in Flow Cytometry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640; Liu, X., et al. (2017). Nat Methods. 14(11):1055-1062; Liu, X., et al. (2020). Nature. 586(7827):101-107).

      Immunocytochemistry Analysis: A representative lot detected NLGN4X in Immunocytochemistry applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).

      ELISA Analysis: A representative lot detected NLGN4X in ELISA applications (O Brien, C.M., et al. (2017). Stem Cells. 35(3):626-640).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant protein fragment corresponding to the extracellular domain of human Neuroligin-4,x-linked (NLGN4X).
      EpitopeExtracellular domain
      CloneCSTEM30
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone CSTEM30 is a mouse monoclonal antibody that detects Neuroligin-4, X-linked (NLGN4X). It targets an epitope within the extracellular domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NLGN4X
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight91.92 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in HFF-hiPSCs.

      Flow Cytometry Analysis (FC): 1 µg of this antibody detected NLGN4X in one million Human foreskin fibroblast-induced pluripotent stem cells (HFF-hiPSCs) .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2301-100UG 04065268900673