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AB1553 Anti-Metabotropic Glutamate Receptor 2/3 Antibody

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AB1553
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, RIH(P), IHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1553
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Metabotropic Glutamate Receptor 2/3 Antibody
      Alternate Names
      • mGluR2/3
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationAffinity purified using the immunogen peptide. Lyophilized from PBS containing 1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Metabotropic Glutamate Receptor 2/3 Antibody detects level of Metabotropic Glutamate Receptor 2/3 & has been published & validated for use in IH(P), IH & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot analysis can be done at final concentrations of 0.1-0.5 μg/mL. AB1553 can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.1-0.5 μg/mL.

      The antibody has not been tested for use in immunoprecipitation. However, antibodies to the carboxy termini of other glutamate receptors are very effective in immunoprecipitation.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenCarboxy terminus peptide of rat mGluR2 conjugated to BSA with glutaraldehyde (NGREVVDSTTSSL).
      HostRabbit
      SpecificityMetabotropic glutamate receptors mGluR2 and mGluR3, which have nearly identical C-termini. There is also a very slight recognition of mGluR5. Western blot analysis of rat brain shows bands migrating at Mr = 100,000 (which contains both mGluR2 and mGluR3) and 190,000. The larger band is thought to be a dimer of the smaller band.
      Species Reactivity
      • Monkey
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryL-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.
      Gene Symbol
      • GRM2
      • mGlu2
      • GPRC1B
      • MGLUR2
      • mGluR2
      • GLUR2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14416 # Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
      SIZE: 872 amino acids; 95568 Da
      SUBUNIT: Interacts with GRASP (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Widely expressed in different regions of the adult brain as well as in fetal brain.
      SIMILARITY: SwissProt: Q14416 ## Belongs to the G-protein coupled receptor 3 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at 2-8°C or -20°C. Reconstitute to 500 μL with sterile distilled water. Store reconstituted material frozen (-20°C or -80°C) in undiluted aliquots for up to six months. Avoid repeated thawing and refreezing. Diluted antibody containing 0.1% sodium azide for Western blot analysis can be stored for several weeks and reused repeatedly.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB1553 04053252325182

      Documentation

      Anti-Metabotropic Glutamate Receptor 2/3 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Metabotropic Glutamate Receptor 2/3 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) - 3307482 3307482
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) - 3903070 3903070
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) - 4162073 4162073
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY 2878312
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY - 2279241 2279241
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY -2584415 2584415
      RABBIT ANTI-METABOTROPIC GLUTAMATE RECEPTOR 2/3 (mGluR2/3) AFFINITY PURIFIED, POLYCLONAL ANTIBODY -2772660 2772660

      References

      Reference overviewApplicationSpeciesPub Med ID
      CCL2-ethanol interactions and hippocampal synaptic protein expression in a transgenic mouse model.
      Gruol, DL; Vo, K; Bray, JG; Roberts, AJ
      Frontiers in integrative neuroscience  8  29  2014

      Show Abstract
      Western Blotting24772072 24772072
      Selective silencing of individual dendritic branches by an mGlu2-activated potassium conductance in dentate gyrus granule cells.
      Brunner, J; Ster, J; Van-Weert, S; Andrási, T; Neubrandt, M; Corti, C; Corsi, M; Ferraguti, F; Gerber, U; Szabadics, J
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  7285-98  2013

      Show Abstract
      23616537 23616537
      Altered synaptic transmission in the hippocampus of transgenic mice with enhanced central nervous systems expression of interleukin-6.
      Nelson, TE; Olde Engberink, A; Hernandez, R; Puro, A; Huitron-Resendiz, S; Hao, C; De Graan, PN; Gruol, DL
      Brain, behavior, and immunity  26  959-71  2012

      Show Abstract
      Western BlottingMouse22609298 22609298
      Neuroadaptive changes in cerebellar neurons induced by chronic exposure to IL-6.
      Gruol, DL; Puro, A; Hao, C; Blakely, P; Janneke, E; Vo, K
      Journal of neuroimmunology  239  28-36  2011

      Show Abstract
      Western BlottingMouse21890220 21890220
      Type 2 metabotropic glutamate receptor (mGluR2) fails to negatively couple to cGMP in stably transfected cells.
      Wroblewska, B; Wegorzewska, IN; Bzdega, T; Neale, JH
      Neurochemistry international  58  176-9  2011

      Show Abstract
      Western Blotting21115084 21115084
      Metabotropic glutamate receptor mGlu2 is resistant to homologous agonist-induced desensitization but undergoes protein kinase C-mediated heterologous desensitization.
      Lennon SM, Rivero G, Matharu A, Howson PA, Jane DE, Roberts PJ, Kelly E
      Eur J Pharmacol  649  29-37. Epub 2010 Sep 15.  2010

      Show Abstract
      20826132 20826132
      Elevated level of metabotropic glutamate receptor 2/3 in the prefrontal cortex in major depression.
      Feyissa, AM; Woolverton, WL; Miguel-Hidalgo, JJ; Wang, Z; Kyle, PB; Hasler, G; Stockmeier, CA; Iyo, AH; Karolewicz, B
      Progress in neuro-psychopharmacology & biological psychiatry  34  279-83  2010

      Show Abstract
      Western Blotting19945495 19945495
      The mGluR2/3 agonist LY379268 blocks the effects of GLT-1 upregulation on prepulse inhibition of the startle reflex in adult rats.
      Bellesi, M; Conti, F
      Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology  35  1253-60  2010

      Show Abstract
      Immunocytochemistry20072121 20072121
      Chronic CXCL10 alters neuronal properties in rat hippocampal culture.
      Cho, J; Nelson, TE; Bajova, H; Gruol, DL
      Journal of neuroimmunology  207  92-100  2009

      Show Abstract
      19167097 19167097
      Group II metabotropic glutamate receptor activation on peripheral nociceptors modulates TRPV1 function.
      Carlton, SM; Du, J; Zhou, S
      Brain research  1248  86-95  2009

      Show Abstract
      Immunohistochemistry19026992 19026992

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies