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MABC954 Anti-MUTYH Antibody, clone 12D5.1

Anti-MUTYH Antibody, clone 12D5.1 is an antibody against MUTYH for use in Western Blotting.

Anti-MUTYH Antibody, clone 12D5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: MUTYH, MYH UniProt Number: Q9UIF7
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MABC954
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC954
      DescriptionAnti-MUTYH Antibody, clone 12D5.1
      Alternate Names
      • A/G-specific adenine DNA glycosylase
      • MutY homolog
      • hMYH
      Background InformationA/G-specific adenine DNA glycosylase (UniProt Q9UIF7; also known as hMYH, MutY homolog) is encoded by the MUTYH (also known as MYH) gene (Gene ID 4595) in human. Base excision repair (BER) is initiated by damage-specific DNA glycosylases that recognize and remove aberrant bases by hydrolyzing the N-glycosidic bond, creating an apurinic/apyrimidinic (AP) site. This is followed by an (AP)-endonuclease-catalyzed DNA strand incision 5′ to the AP site, trimming of the 5′ end by an (AP)-lyase or an endonuclease, DNA resynthesis by a DNA polymerase and strand sealing by a DNA ligase. Monofunctional glycosylases have only glycosylase activity, whereas bifunctional glycosylases possess additional AP lyase activity and can therefore convert a base lesion into a single-strand break without the need for a separate AP endonuclease. MUTYH/MYH is a monofunctional DNA glycosylase that excises mismatched adenine opposite 7, 8-dihydro-8-oxoguanine (8-oxoG), which is the major mutagenic lesion induced by oxidative stress. Biallelic mutations in MUTYH are associated with MUTYH-Associated polyposis (MAP) and increased risk in colorectal cancer (CRC).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MUTYH Antibody, clone 12D5.1 is an antibody against MUTYH for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant internal fragment from the N-terminal half of human MUTYH.
      Epitope N-terminal half.
      Clone12D5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all six spliced isoforms of human MUTYH reported by UniProt (Q9UIF7).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MUTYH
      • MYH
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~59 kDa observed. 60.07 kDa (isoform Alpha-1), 59.14 kDa (isoform Alpha-2), 59.07 kDa (isoform Alpha-3), 58.44 kDa (isoform Beta-1), 57.52 kDa (isoform Gamma-2), and 50.52 kDa (isoform Gamma-3) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected MUTYH in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC954 04055977331219