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MABT837 Anti-LBPA Antibody, clone 6C4

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MABT837
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, EM, ELISA, DBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT837
      DescriptionAnti-LBPA Antibody, clone 6C4
      Alternate Names
      • LBPA
      Background InformationLyso bis-phosphatidic acid (LBPA, Lysobisphosphatidic acid, L(bis)PA) is a lipid that is exclusively located in late endosomes and is used as a late endosome marker. One of the characteristic features of late endosomes is a complex system of internal membranes within the lumen that contains large amounts of the unique, poorly degradable LBPA, and thus forms a specialized membrane domain within endosomes. Late endosomes function not only as a major protein-sorting compartment, but also an obligatory station for LDL and other endocytosed ligands destined for degradation. Research shows that the LBPA-rich membranes within late endosomes regulate cholesterol transport, presumably by acting as a collection and distribution device. The genetic disease Niemann–Pick type C (NPC) is characterized by both lysosomal and endosomal storage disorders. In skin fibroblasts from NPC patients, cholesterol accumulation is seen within vesicles containing late endosome markers LBPA and Rab7.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-LBPA Antibody, clone 6C4 is validated for use in Immunocytochemistry, Electron Microscopy, ELISA and Dot Blot for the detection of LBPA.
      Key Applications
      • Immunocytochemistry
      • Electron Microscopy
      • ELISA
      • Dot Blot
      Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected LBPA in A431, HUVEC and NIH/3T3 cells.
      Immunocytochemistry Analysis: A representative lot detected late endosome LBPA immunoreactivity by fluorescent immunocytochemistry using MLN64-overexpressing MCF7 cells (Alpy, F., et al. (2001). J Biol Chem. 276(6):4261-4269).
      Immunocytochemistry Analysis: A representative lot detected late endosome LBPA immunoreactivity in skin fibroblasts from healthy donors, as well as Niemann–Pick type C (NPC) and Tay-Sachs disease patients by fluorescent immunocytochemistry (Kobayahsi, T., et al. (1999). Nat Cell Biol. 1(2):113-118).
      Immunocytochemistry Analysis: A representative lot detected late endosome LBPA immunoreactivity in baby hamster kidney (BHK) cells by fluorescent immunocytochemistry (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
      Electron Microscopy Analysis: A representative lot detected similar cellular LBPA distribution in the skin fibroblasts from autosomal recessive Niemann–Pick type C disease (NPC) patients as seen in baby hamster kidney (BHK) cells (Kobayahsi, T., et al. (1999). Nat Cell Biol. 1(2):113-118).
      Electron Microscopy Analysis: A representative lot detected LBPA immunoreactivity specifically associated with the late endosomes in baby hamster kidney (BHK) cells, but not the lgp120-positive limiting membranes of the same endosomes (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
      ELISA Analysis: A representative lot specifically detected wells coated with total baby hamster kidney (BHK) cell lipid extract or purified LBPA, but not other lipids (PC, PE, SM, PS, CL, or PI) purified BHK lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197)
      Dot Blot Analysis: A representative lot specifically detected purified LBPA spotted on a HPTLC plate, but not other lipids (PC, PE, SM, PS, CL, PI, Sul, Chol, Cer, CE) purified from baby hamster kidney (BHK) cell lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
      Biological Information
      ImmunogenEndosomal membranes from baby hamster kidney fibroblasts (BHK) corresponding to Hamster LBPA.
      Clone6C4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6C4 specifically detects LBPA, but not PC, PE, SM, PS, CL, PI, Sul, Chol, Cer, or CE purified from baby hamster kidney (BHK) cell lipid extract (Kobayahsi, T., et al. (1998). Nature. 392(6672):193-197).
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteAll Species. Target molecule is not species-specific.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: 4.0 µg/mL of this antibody stained late endosomes in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT837 04055977294200

      Documentation

      Anti-LBPA Antibody, clone 6C4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-LBPA Antibody, clone 6C4 Certificates of Analysis

      TitleLot Number
      Anti-LBPA, clone 6C4 - 2950529 2950529
      Anti-LBPA, clone 6C4 - 3192013 3192013
      Anti-LBPA, clone 6C4 - 3204832 3204832
      Anti-LBPA, clone 6C4 - 3268874 3268874
      Anti-LBPA, clone 6C4 - 3419572 3419572
      Anti-LBPA, clone 6C4 - 3508318 3508318
      Anti-LBPA, clone 6C4 - 3682527 3682527
      Anti-LBPA, clone 6C4 - 3793685 3793685
      Anti-LBPA, clone 6C4 - 3916722 3916722
      Anti-LBPA, clone 6C4 - 3990927 3990927

      References

      Reference overviewPub Med ID
      The steroidogenic acute regulatory protein homolog MLN64, a late endosomal cholesterol-binding protein.
      Alpy, F; Stoeckel, ME; Dierich, A; Escola, JM; Wendling, C; Chenard, MP; Vanier, MT; Gruenberg, J; Tomasetto, C; Rio, MC
      The Journal of biological chemistry  276  4261-9  2001

      Show Abstract
      11053434 11053434
      Late endosomal membranes rich in lysobisphosphatidic acid regulate cholesterol transport.
      Kobayashi, T; Beuchat, MH; Lindsay, M; Frias, S; Palmiter, RD; Sakuraba, H; Parton, RG; Gruenberg, J
      Nature cell biology  1  113-8  1999

      Show Abstract
      10559883 10559883
      A lipid associated with the antiphospholipid syndrome regulates endosome structure and function.
      Kobayashi, T; Stang, E; Fang, KS; de Moerloose, P; Parton, RG; Gruenberg, J
      Nature  392  193-7  1998

      Show Abstract
      9515966 9515966

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