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MABC60 Anti-KiSS-1 Antibody, clone 8H4.1

Anti-KiSS-1 Antibody, clone 8H4.1 is an antibody against KiSS-1 for use in IHC, Western Blotting.

Anti-KiSS-1 Antibody, clone 8H4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Cytoskeleton Gene Symbol: KISS1, PP5098 UniProt Number: Q15726
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MABC60
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC60
      DescriptionAnti-KiSS-1 Antibody, clone 8H4.1
      Alternate Names
      • Metastasis-suppressor KiSS-1
      • Kisspeptin-1
      • Metastin
      • Kisspeptin-542
      • Kisspeptin-143
      • Kisspeptin-134
      • Kisspeptin-10
      Background InformationMetastasis-suppressor KiSS-1 (KiSS-1; or Kisspeptin-1) is one of several endogenous ligands for the orphan G-protein coupled receptor GPR54. KiSS-1 functions as a suppressor of metastasis in various types of cancers, by inhibiting migration and proliferation in affected cells. This effect may be produced by the activation of multiple signaling pathways: Ligand-bound GPR54 activates IP3 and PKC signaling via a PLC pathway; and ERK 1 and ERK 2 by a cPLA2, which may lead to increased calcium signaling and gene transcription, respectively. More recently, however, KiSS-1/GPR54 signaling has been implicated in puberty and development. KiSS-1/GPR54 may regulate signaling of GnRH neurons during puberty and may influence the release of FH and LSH hormones. KISS-1 is highly expressed in the CNS, testis, pancreas, intestine, ovary, and placenta.
      References
      Product Information
      FormatPurified
      Control
      • Human placenta tissue
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-KiSS-1 Antibody, clone 8H4.1 is an antibody against KiSS-1 for use in IHC, Western Blotting.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected KiSS-1 in human pancreas and rat testis tissues.
      Biological Information
      ImmunogenRecombinant protein corresponding to human KiSS-1.
      Clone8H4.1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHunan, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • KISS1
      • PP5098
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca2+ levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.

      SUBCELLULAR LOCATION: Secreted.

      TISSUE SPECIFICITY: Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast.

      INDUCTION: Down-regulated during the progression of melanoma in vivo. Diminishes MMP9 expression by effecting reduced NF-kappa-B binding to the promoter.

      PTM: Processed by MMP2 and MMP9.

      SEQUENCE SIMILARITIES: Belongs to the KISS1 family.

      SEQUENCE CAUTION: The sequence AAC79512.1 differs from that shown. Reason: Frameshift at position 138.

      The sequence AAM78592.1 differs from that shown. Reason: Frameshift at position 138.
      Molecular Weight15 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human placenta tissue.

      Immunohistochemistry Analysis: 1:1,000 dilution of this antibody detected KiSS-1 in human placenta tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC60 04053252595967

      Documentation

      Anti-KiSS-1 Antibody, clone 8H4.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-KiSS-1 Antibody, clone 8H4.1 Certificates of Analysis

      TitleLot Number
      Anti-KiSS-1, clone 8H4.- 1 3190924 3190924
      Anti-KiSS-1, clone 8H4.1 - 2394059 2394059
      Anti-KiSS-1, clone 8H4.1 - 2420490 2420490
      Anti-KiSS-1, clone 8H4.1 - 2442559 2442559
      Anti-KiSS-1, clone 8H4.1 - 2468178 2468178
      Anti-KiSS-1, clone 8H4.1 - 2829149 2829149
      Anti-KiSS-1, clone 8H4.1 - 3252115 3252115
      Anti-KiSS-1, clone 8H4.1 - 3384528 3384528
      Anti-KiSS-1, clone 8H4.1 - 3597299 3597299
      Anti-KiSS-1, clone 8H4.1 - 3899581 3899581
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      Brochure

      Title
      New Products: Volume 3, 2012