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MAB1611 Anti-Keratin Epithelial Antibody, clone AE3

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MAB1611
500 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, Ch, H, M, Mk, R, RbWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1611
      Replaces04-588
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Keratin Epithelial Antibody, clone AE3
      Alternate Names
      • Type II Cytokeratin
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: MCF-7, RT112, and A431 cell lines.
      PresentationLiquid in PBS. Contains 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Keratin Epithelial Antibody, clone AE3 is an antibody against Keratin Epithelial for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry on unfixed frozen sections or formalin-fixed paraffin-embedded tissue sections. For paraffin-embedded sections, antigen retrieval (HEIR or trypsin treatment) is strongly recommended.

      Western blotting: Clone AE3 recognizes the 65 to 67, 64, 59, 58, 56 and 52 kDa human cytokeratins of the basic subfamily.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenHuman epidermal cells
      CloneAE3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes most of the basic (Type II) keratins, HMW cytokeratins. It is broadly reactive and stains positively almost all epithelia and their neoplasms. AE3 reacts with all layers of epidermis (basal layer and above). The antibody is an excellent marker for distinguishing carcinomas from non-epithelial tumours. It has been used for studying the immunolocalisation of epidermal keratins, tissue distribution of keratins, various diseases of epidermis, oral mucosa and other epithelia and other epithelia development. AE3 and AE1 (Catalog Number MAB1612) have been used extensively for distinguishing carcinoma from non-carcinoma.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Chicken
      • Human
      • Mouse
      • Monkey
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT8
      • KO
      • K8
      • CYK8
      • Keratin-8
      • CK-8
      • Cytokeratin-8
      • K2C8
      • CK8
      • CARD2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05787 # Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
      SIZE: 483 amino acids; 53704 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
      PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
      DISEASE:SwissProt: P05787 # Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].
      SIMILARITY: SwissProt: P05787 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size500 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1611 04053252614323