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MABS2307-25UL Anti-JMY Antibody, clone HMY 117

Anti-JMY, clone HMY 117, Cat. No. MABS2307, is a mouse monoclonal antibody that detects Junction-mediating and -regulatory protein (JMY) and is tested for use in Immunohistochemistry and Western Blotting.

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MABS2307-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2307-25UL
      DescriptionAnti-JMY Antibody, clone HMY 117
      Alternate Names
      • Junction-mediating and -regulatory protein
      Background InformationJunction-mediating and -regulatory protein (UniProt: Q8N9B5; also known as JMY) is encoded by the JMY gene (Gene ID: 133746) in human. JMY is involved in a complex pathway connecting cell motility and invasion with the p53 response to DNA damage. It is widely expressed in many types of tissue- with cytoplasmic and nuclear co-expression. It contains a unique N-terminal domain (aa 1-126) that is essential for its interaction with p300/EP300; three central coiled-coil segments (aa 324-360; 489-541; and 590-621), a polyproline region (aa 801-830), and a C-terminal WH2 domain (aa 921-938). It localizes primarily to the nucleus, but is also found at the leading edge in migrating cells. It acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In the nucleus, it acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300 and increases p53/TP53-dependent transcription and apoptosis. In the cytoplasm, it acts as a nucleation-promoting factor for both branched and unbranched actin filaments. It promotes the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. However, it does not affect elongation of preformed filaments. JMY is shown to be expressed in every type of tumor investigated. Overexpression of JMY in human U2OS cells is shown to induce the formation of elongated actin filament structures. JMY is ubiquitinated by MDM2 that leads to its proteasomal degradation. In case of DNA damage its interaction with MDM2 is altered that prevents its degradation and allows interaction with p300/EP300. (Ref.: Adighibe, O., et al. (2014). Virchows Arch. 465(6); 715-722; Firat-Karalar, EN., et al. (2011). Mol. Biol. Cell. 22(23); 4563-4574; Zuchero, JB., et al. (2009). Nat. Cell Biol. 11(4); 451-459).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-JMY, clone HMY 117, Cat. No. MABS2307, is a mouse monoclonal antibody that detects Junction-mediating and -regulatory protein (JMY) and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected JMY in Immunohistochemistry applications (Adighibe, O., et al. (2014). Virchows Arch. 465(6):715-22).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated three peptides corresponding to 13 amino acids from the N-terminal region, 14 amino acids from the internal region, and 23 amino acids from the C-terminal region of human Junction-mediating and -regulatory protein (JMY).
      EpitopeUnknown
      CloneHMY 117
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone HMY 117 is a mouse monoclonal antibody that detects Junction-mediating and -regulatory protein (JMY).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • JMY
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~130 kDa observed; 111.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Junction-mediating and -regulatory protein (JMY) in HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS2307-25UL 04065268041949