Millipore Sigma Vibrant Logo

CBL1304 Anti-Integrin α4 Antibody, clone PS/2

View This Product on Sigma-Aldrich
CBL1304
500 µg  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MFC, IF, IP, IHC, FUNCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL1304
      Replaces04-1131
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α4 Antibody, clone PS/2
      Alternate Names
      • CD49d
      • VLA-4
      Background InformationVirtually all lymphoid and myeloid cells in bone marrow are reactive with the anti-VLA-4 antibody. VLA-4 is absent or minimally detectable on stromal cells. Myeloid cells maintained in Dexter-type long-term cultures of murine bone marrow are positive, as are lymphoid cells from Whitlock-White cultures.
      Product Information
      FormatPurified
      PresentationPurified rat IgG2bκ in 0.01 M phosphate buffer, pH 7.1 containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Integrin α4 Antibody, clone PS/2 detects level of Integrin α4 & has been published & validated for use in FC, IF, IP, IH.
      Key Applications
      • Flow Cytometry
      • Immunofluorescence
      • Immunoprecipitation
      • Immunohistochemistry
      • Affects Function
      Application NotesFlow cytometry: 2 μg per 106 cells

      Immunohistochemistry on frozen tissue sections

      Immunoprecipitation

      Cell adhesion assays

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ClonePS/2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityClone PS/2 recognises the murine alpha4 chain of the VLA-4 integrin heterodimer, 150 kDa. The antibody also reacts with human VLA-4.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.
      Gene Symbol
      • ITGA4
      • IA4
      • MGC90518
      • VLA-4
      • CD49D
      • CD49d
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13612 # Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha- 4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha- 4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
      SIZE: 1038 amino acids; 115333 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P13612 The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion.
      PTM: Phosphorylation on Ser-1027 inhibits PXN binding.
      SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20ºC for up to 12 months from date of receipt.Upon first use, aliquot into smaller quantities to avoid additional freeze/thaw cycles.
      Packaging Information
      Material Size500 µg
      Global Trade Item Number
      Catalogue Number GTIN
      CBL1304 04053252625169