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MABF2778-25UL Anti-IRF-3 Antibody, clone AR-1

Anti-IRF-3, clone AR-1, Cat. No. MABF2778, is a mouse monoclonal antibody that detects Interferon regulatory factor 3 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

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MABF2778-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2778-25UL
      DescriptionAnti-IRF-3 Antibody, clone AR-1
      Alternate Names
      • Interferon regulatory factor 3
      Background InformationInterferon regulatory factor 3 (UniProt: Q14653; also known as IRF-3) is encoded by the IRF3 gene (Gene ID: 3661) in human. IRF-3 is a transcription factor that is constitutively expressed in a variety of tissues and plays a role in intracellular immune response against DNA and RNA viruses. It regulates the transcription of type I interferon genes (IFN-a and IFN-b) and interferon-stimulated genes by binding to an interferon-stimulated response element in their promoters. IRF-3 contains a DNA-binding domain (aa 5-111), nuclear export signal (aa 139-149), IRF-interacting domain, and a C-terminal serine-rich region that contains several phosphorylation sites. Although some of these serines are shown to be phosphorylated in the resting state, further phosphorylation of specific residues is required for its activation and nuclear translocation. In uninfected cells, IRF-3 is present in the cytoplasm, however, following infection, it undergoes phosphorylation on serine 385, serine 386, threonine 390, and serine 396. These phosphorylation events induce a conformational change that leads to its dimerization and nuclear localization where it associates with CREB binding protein to form dsRNA activated factor 1 that subsequently leads to the activation of type I interferons and ISG genes. Phosphorylation and subsequent activation of IRF-3 shown to be inhibited by vaccinia virus protein E3. Mutations in IRF3 gene have been linked to infection-induced, Herpes-specific acute encephalopathy that is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Clone AR-1 recognizes an epitope within the DNA-binding domain of IRF-3. It can detect resting IRF-3 as well as the activated form. (Ref.: Rustagi, A., et al. (2013). Methods. 59(2); 225-232; Honda, K., et al. (2006). Immunity. 25(3); 349-360).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IRF-3, clone AR-1, Cat. No. MABF2778, is a mouse monoclonal antibody that detects Interferon regulatory factor 3 and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • ELISA
      • Flow Cytometry
      Application NotesTested Applications

      ELISA Analysis: A representative lot detected IRF-3 in ELISA applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

      Flow Cytometry Analysis: A representative lot detected IRF-3 in Flow Cytometry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

      Western Blotting Analysis: A representative lot detected IRF-3 in Western Blotting applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

      Immunocytochemistry Analysis: A representative lot detected IRF-3 in Immunocytochemistry applications (Rustagi, A., et. al. (2013). Methods. 59(2):225-32).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged full-length human recombinant human Interferon regulatory factor 3 (IRF-3).
      EpitopeN-terminal half
      CloneAR-1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone AR1 is a mouse monoclonal antibody that detects Interferon regulatory factor 3 (IRF-3). It targets an epitope within the DNA-binding domain from the N-terminal half.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rhesus Macaque
      Species Reactivity NoteHuman, Monkey.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRF3
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~51 kDa observed; 47.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-3 in A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2778-25UL 04065266553864