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MABF2315-100UG Anti-IDO-2 Antibody, clone 13-3

Anti-IDO-2, clone 13-3, Cat. No. MABF2315, is a mouse monoclonal antibody that detects Indoleamine 2,3-dioxygenase 2 and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MABF2315-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2315-100UG
      DescriptionAnti-IDO-2 Antibody, clone 13-3
      Alternate Names
      • Indoleamine 2
      • 3-dioxygenase 2
      • Indoleamine 2
      • 3-dioxygenase-like protein 1
      • Indoleamine-pyrrole 2
      • 3-dioxygenase-like protein 1
      Background InformationIndoleamine 2,3-dioxygenase 2 (UniProt: Q6ZQW0; also known as IDO-2, Indoleamine 2,3-dioxygenase-like protein 1, Indoleamine-pyrrole 2,3-dioxygenase-like protein 1) is encoded by the IDO2 (also known as INDOL1) gene (Gene ID: 169355) in human. IDO-2 is detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. In testis and thyroid, it is expressed at low levels. It catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. IDO-1 and IDO-2 are 2 distinct enzymes which catalyze the same reaction. However, the Km for IDO-2 for tryptophan is much higher than that of IDO-1. IDO-2 may play a role as a negative regulator of IDO-1 by competing for heme-binding with IDO-1. IDO-2 activity is inhibited by 1MT (1-methyl-tryptophan) and MTH-trp (methylthiohydantoin-DL-tryptophan). IDO-2 is also involved in immune regulation. IDO-2 knockout mice do not show any apparent defects in their embryonic development or hematopoietic differentiation and have wild-type profiles for kynurenine in blood serum and for immune cells in spleen, lymph nodes, peritoneum, thymus and bone marrow. However, the knockout mice exhibit defects in IDO-mediated T-cell regulation and inflammatory responses. (Ref.: Mbongue JC, et al. (2015). Vaccines (Basel). 3(3); 703-729).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-IDO-2, clone 13-3, Cat. No. MABF2315, is a mouse monoclonal antibody that detects Indoleamine 2,3-dioxygenase 2 and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected IDO-2 in human spleen and human liver tissue sections.

      Immunoprecipitation Analysis: A representative lot detected IDO-2 in Human and mouse IDO-2 T-Rex extracts (Contributed from the group of Lisa Laury-Kleintop, Ph.D., Lankenau Institute for Medical Research, USA).

      Western Blotting Analysis: A representative lot detected IDO-2 in Human and Mouse IDO-2 T-Rex cell lysates (Contributed from the group of Lisa Laury-Kleintop, Ph.D., Lankenau Institute for Medical Research, USA).
      Biological Information
      ImmunogenKLH-conjugated peptide corresponding to 21 amino acids from the C-terminal half of human Indoleamine 2,3-dioxygenase 2 (IDO2).
      Clone13-3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 13-3 is a mouse monoclonal antibody that detects Indoleamine 2,3-dioxygenase 2 (IDO-2). It targets an epitope within 21 amino acids from the C-terminal half.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IDO2
      • INDOL1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~57 kDa observed; 47.07 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human Placenta tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected IDO-2 in Human Placenta tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2315-100UG 04061841369509