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ABE403 Anti-Histone H3.3 G34R

Anti-Histone H3.3 G34R, Cat. No. ABE403, is a highly specific rabbit polyclonal antibody that targets Histone H3.3 mutant G34R and has been tested for use in Immunohistochemistry and Western Blotting.

Anti-Histone H3.3 G34R MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: H3.3A, H3F3, PP781, H3F3B, H3.3B UniProt Number: P84243
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ABE403
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE403
      DescriptionAnti-Histone H3.3 G34R
      Alternate Names
      • H3.3
      Background InformationHistone H3.3 (UniProt: P84243) is encoded by the H3FA3 (also known as H3.3A, H3F3, PP781, H3F3B, H3.3B) gene (Gene ID: 3020) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H3.3 is expressed throughout the cell cycle independently of DNA synthesis. It is a variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. It is the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. While H3.3 can function much the same as canonical H3 as a core part of the nucleosome, H3.3 is also deposited into transcriptionally active regions to replace displaced nucleosomes throughout the cell cycle. In tumor cells, H3.3K27M and H3.3G34R/V mutations are heterozygously expressed, with one allele of H3F3A being wildtype. K27M and G34R/V mutations are mutually exclusive in tumors and display distinct gene expression profiles and DNA methylation patterns. Almost all tumors bearing G34R/V mutations also exhibit mutations in ATRX/DAXX and display alternate lengthening of telomeres, which is a classical phenotype of cancerous cells. (Ref.: Yuen, BT., and Knoepfler PS (2013). Cancer cell 24(5); 567-74).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Histone H3.3 G34R, Cat. No. ABE403, is a highly specific rabbit polyclonal antibody that targets Histone H3.3 mutant G34R and has been tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenConjugated linear peptide correspding to 8 amino acids from the N-terminal region of human histone H3.3 with a G34R mutation.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects G34R mutated form of human Histone H3.3. It does not detect widl-type Histone 3.3.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H3.3A
      • H3F3
      • PP781
      • H3F3B
      • H3.3B
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~17 kDa observed; 15.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Hek293 cells transfected with Histone 3.3 G34R mutant.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Histone H3.3 G34R in Hek293 cells transfected with Histone 3.3 G34R mutant.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE403 04054839402043