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ABE1302 Anti-Hec1 Antibody

Anti-Hec1 Antibody is an antibody against Hec1 Antibody for use in Western Blotting, Immunocytochemistry.

Anti-Hec1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NDC80, HEC, HEC1, KNTC2 UniProt Number: O14777
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ABE1302
50 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1302
      DescriptionAnti-Hec1 Antibody
      Alternate Names
      • Kinetochore protein NDC80 homolog
      • Highly expressed in cancer protein
      • Kinetochore protein Hec1
      • HsHec1
      • Kinetochore-associated protein 2
      • Retinoblastoma-associated protein HEC
      • Hec1
      Background InformationThe protein HEC1 (highly expressed in cancer 1) is an essential component of the vital NDC80 kinetochore-associated protein complex. The NDC80 complex is required for chromosome segregation and spindle checkpoint activity. HEC1 is essential for proper kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. HEC1 is expressed by all dividing cells and is particularly high in cancers. HEC1 localizes to kinetochores from late prophase to anaphase, specifically at the outer plate of the kinetochore. HEC1 is normally phosphorylated during its active phase at multiple sites and the phosphorylation appears to tune kinetochore-microtubule binding affinity during mitosis. Moreover, recent research using small molecule inhibitors to HEC1's kinase, Nek2, demonstrate that phosphorylation is required for proper HEC1 function.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Hec1 Antibody is an antibody against Hec1 Antibody for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1.0 µg/mL from a representative lot detected Hec1 in HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the Nuclear localization region of human Hec1.
      EpitopeNuclear localization region
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NDC80
      • HEC
      • HEC1
      • KNTC2
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~78 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SK-OV-3 cell lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected Hec1 in 10 µg of SK-OV-3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1302 04055977127317