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MABC1035 Anti-Gremlin-1 Antibody, clone 2H1.1

Anti-Gremlin-1, clone 2H1.1, Cat. No. MABC1035, is a mouse monoclonal antibody that detects Gremlin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Gremlin-1 Antibody, clone 2H1.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: GREM1, CKTSF1B1, DAND2, DRM, PIG2 UniProt Number: O60565
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MABC1035
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1035
      DescriptionAnti-Gremlin-1 Antibody, clone 2H1.1
      Alternate Names
      • Cell proliferation-inducing gene 2 protein
      • Cysteine knot superfamily 1
      • BMP antagonist 1
      • DAN domain family member 2
      • Down-regulated in Mos-transformed cells protein
      • Increased in high glucose protein 2
      • IHG-2
      Background InformationGremlin-1 (UniProt: O60565; also known as Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2, IHG-2) is encoded by the GREM1 (also known as CKTSF1B1, DAND2, DRM, PIG2) gene (Gene ID: 26585 ) in human. Gremlin-1 is a homodimeric cytokine that plays an important role during carcinogenesis and metanephric kidney organogenesis. Two isoforms of Gremlin-1 have been described that are produced by alternative splicing. Gremlin-1 acts as an antagonist of BMP2 and inhibits BMP2-mediated differentiation of osteoblasts. It also down-regulates the BMP4 signaling in a dose-dependent manner. Gremlin-1 is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off. Gremlin-1 is highly expressed in small intestine where its expression is restricted to intestinal subepithelial myofibroblasts (ISEMFs) at the crypt base. It is also expressed in fetal brain and colon and weak expression is observed in adult brain ovary, prostate, pancreas, and skeletal muscle. Its levels are down-regulated in tumor cell lines. Mutations in GREM1 gene are known to cause Polyposis syndrome, mixed hereditary 1 (HMPS1) that is characterized by apparent autosomal dominant inheritance of multiple types of colorectal polyp, with colorectal carcinoma occurring in a high proportion of affected individuals.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Gremlin-1, clone 2H1.1, Cat. No. MABC1035, is a mouse monoclonal antibody that detects Gremlin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Gremlin-1 in human prostate and human colon tissues.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 89 amino acids from the internal region.
      Clone2H1.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2H1.1 detects Gremlin-1 in human cells. It targets an epitope within 89 amino acids from the internal region of isoform 1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GREM1
      • CKTSF1B1
      • DAND2
      • DRM
      • PIG2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~36 kDa observed; 20.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Gremlin recombinant protein.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Gremlin-1 recombinant protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1035 04054839415272