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MABS1212 Anti-Geminin Antibody, clone 4B10.1

Detect Geminin using this mouse monoclonal Anti-Geminin, clone 4B10.1 Antibody, Cat. No. MABS1212, validated for use in Immunocytochemistry and Western Blotting.

Anti-Geminin Antibody, clone 4B10.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: GMNN UniProt Number: O75496
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MABS1212
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1212
      DescriptionAnti-Geminin Antibody, clone 4B10.1
      Alternate Names
      • Geminin
      Background InformationGeminin (UniProt O75496) is encoded by the GMNN gene (Gene ID 51053) in human. Geminin is a negative regulator of DNA-replication and the founding member of the Geminin family, whose members also include Idas (multicilin; McIdas) and GemC1 (Geminin coiled-coil domain-containing protein 1). Geminin binds Cdt1 and inhibits the mini-chromosome maintenance complex (MCM) loading onto chromatin and pre-replication complex (preRC) formation. Geminin is a 209-amino acid protein that can form homodimer or heterodimers with either Idas or GemC1 via the middle coiled coil structure (a.a. 94-144 of Geminin) in a head-to-tail fashion. The same region in Geminin (a.a. 82-161) is also essential for Cdt1 interaction. A homeodomain-binding domain is located at the C-terminal region of Geminin (a.a. 170-190). GemC1 is also implicated in DNA replication but at a later stage than Geminin. All three Geminin family members play important roles in cell proliferation and differentiation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Geminin using this mouse monoclonal Anti-Geminin, clone 4B10.1 Antibody, Cat. No. MABS1212, validated for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a representative lot detected nuclear geminin immunoreactivity in HUVECs, A431, and HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant human geminin internal fragment.
      Clone4B10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4B10.1 detects human geminin by targeting an internal epitope.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GMNN
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~31 kDa observed. 23.57 kDa calculated. The larger-than-predicted band size is consistent with that reported in the literature (McGarry, T.J., and Kirschner, M.W. (1998). Cell. 93(6):1043-1053 ). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected geminin in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1212 04054839049736