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MABC1096-25UG Anti-GEF-H1 Antibody, clone 3C5

Anti-GEF-H1, clone 3C5, Cat. No. MABC1096, is a mouse monoclonal antibody that detects Rho guanine nucleotide exchange factor 2 (GEF-H1)and has been tested for use in Western Blotting.

MABC1096-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1096-25UG
      DescriptionAnti-GEF-H1 Antibody, clone 3C5
      Alternate Names
      • Rho guanine nucleotide exchange factor 2
      • Guanine nucleotide exchange factor H1
      • Microtubule-regulated Rho-GEF
      • Proliferating cell nucleolar antigen p40
      Background InformationRho guanine nucleotide exchange factor 2 (UniProt: Q92974; also known as Guanine nucleotide exchange factor H1, GEF-H1, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40) is encoded by the ARHGEF2 (also known as KIAA0651, LFP40) gene (Gene ID: 9181) in human. GEF-H1 is a microtubule-associated guanine nucleotide exchange factor (GEF) for the Rho family of small GTPases that activates Rho-GTPases by promoting the exchange of GDP for GTP. It localizes to the tips of cortical microtubules of the mitotic spindle during cell division and is released upon microtubule depolymerization. GEF-H1 is involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and innate immune response. GEF-H1 can act as an adaptor protein that links PP2A B subunits to KSR-1 and mediate the dephosphorylation of KSR-1 serine 392 and activate MAPK signaling. GEF-H1 I known to contributes to cell survival and growth in HRASV12- transformed cells and is reported to be essential for activation of MAP kinase pathway in response to HRASV12. It is also reported to be important for the growth and survival of HRASV12 transformed cells. GEF-H1 contains a DH (DBL-homology) domain (aa 235-432) that interacts with and promotes loading of GTP on RhoA and tyrosine phosphorylation of RIPK2. It also has two phorbol-ester/DAG-type zinc finger (aa 587-611) and the C-terminal coiled coil domains (aa 798-867), both of which are required for association with microtubules. (Ref.: Cullis, J., et al. (2014). Cancer Cell 25(2); 181-195).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-GEF-H1, clone 3C5, Cat. No. MABC1096, is a mouse monoclonal antibody that detects Rho guanine nucleotide exchange factor 2 (GEF-H1)and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected GEF-H1 in Western Blotting applications (Cullis, J., et. al. (2014). Cancer Cell. 25(2):181-95).
      Biological Information
      ImmunogenA linear peptide from the N-terminal region of human GEF-H1
      Clone3C5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3C5 is a mouse monoclonal antibody that specifically detects human Rho guanine nucleotide exchange factor 2 (GEF-H1).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ARHGEF2
      • KIAA0651
      • LFP40
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~111 kDa observed; 111.54 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates from untreated HCT116 cells and those pretreated with MEK inhibitors PD98059 and U0126.

      Western Blotting Analysis: 2 µg/mL of this antibody detected GEF-H1 in lysates from untreated HCT116 cells, but not in cells pretreated with MEK inhibitors PD98059 and U0126.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1096-25UG 04054839997303

      Documentation

      Anti-GEF-H1 Antibody, clone 3C5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GEF-H1 Antibody, clone 3C5 Certificates of Analysis

      TitleLot Number
      Anti-GEF-H1, clone 3C5 - 3239909 3239909

      References

      Reference overviewPub Med ID
      The RhoGEF GEF-H1 is required for oncogenic RAS signaling via KSR-1.
      Cullis, J; Meiri, D; Sandi, MJ; Radulovich, N; Kent, OA; Medrano, M; Mokady, D; Normand, J; Larose, J; Marcotte, R; Marshall, CB; Ikura, M; Ketela, T; Moffat, J; Neel, BG; Gingras, AC; Tsao, MS; Rottapel, R
      Cancer Cell  28  181-98  2017

      Show Abstract       		24525234 24525234