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MAB10207-I-25UL Anti-Fusion Glycoprotein of HPIV 3 Antibody, clone 9-4-3

Anti-Fusion Glycoprotein of HPIV 3, clone 9-4-3, Cat. No. MAB10207-I, is a mouse monoclonal antibody that detects human parainfluenza type 3 virus and is tested for use in Immunocytochemistry and Western Blotting.

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MAB10207-I-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB10207-I-25UL
      DescriptionAnti-Fusion Glycoprotein of HPIV 3 Antibody, clone 9-4-3
      Alternate Names
      • Fusion glycoprotein F0
      Background InformationFusion glycoprotein F0 (UniProt: P06828) is encoded by the F gene (Gene ID: 2828653) by the Human parainfluenza 3 virus (HPIV-3). HPIVs are antigenically divided into types 1-4 and type 1 and 3 are reported to cause most lower respiratory infections. HPIVs are enveloped and of medium size (150 to 250 nm), and their RNA genome is in the negative sense. HPIV3 displays three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and plasma cell membrane fusion, the heptad repeat (HR) regions assume a trimer-of-hairpins structure, positioning the fusion peptide near the C-terminal region of the ectodomain. The formation of this structure drives apposition and subsequent fusion of viral and plasma cell membranes. This fusion is reported to be pH independent and occurs directly at the outer cell membrane. Fusion glycoprotein F0 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. This inactive precursor F0 is glycosylated and proteolytically cleaved into F1 (aa 110-539) and F2 (19-109) to be functionally active. The cleavage is mediated by cellular proteases during the transport and maturation of the polypeptide. (Ref.: Hendrickson, KJ. (2003). Clin. Microbiol. Rev. 16(2); 242-264).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Fusion Glycoprotein of HPIV 3, clone 9-4-3, Cat. No. MAB10207-I, is a mouse monoclonal antibody that detects human parainfluenza type 3 virus and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Fusion Glycoprotein of HPIV-3 in Western Blotting applications (Xie, W., et. al. (2015). PLoS One. 10(8):e0136474).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFusion (F) glycoprotein of human parainfluenza type 3 virus.
      EpitopeUnknown
      Clone9-4-3
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 9-4-3 is mouse monoclonal antibody that detects Fusion glycoprotein of Parainfluenza 3 virus.
      IsotypeIgG1
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • F
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight60.06 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in LLC-ML2 cells infected with HPIV-3.

      Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Fusion Glycoprotein of HPIV 3 in LLC-ML2 cells infected with HPIV-3.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB10207-I-25UL 04065265944915