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ABN1038 Anti-Fbxo7 Antibody

Anti-Fbxo7 Antibody is an antibody against Fbxo7 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry.

Anti-Fbxo7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Signaling Gene Symbol: FBXO7, FBX7 UniProt Number: Q9Y3I1
ABN1038
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHC, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1038
      DescriptionAnti-Fbxo7 Antibody
      Alternate Names
      • F-box only protein 7
      • Fbxo7
      Background InformationF-box only protein 7 (UniProt Q9Y3I1) is encoded by the FBXO7 (Also known as FBX, FBX7, PKPS, FBX07, PARK15) gene in human (Gene ID 25793). The Skp1-Cul1-F box protein (SCF) E3 ubiquitin ligase complexes rely on their F-box protein component for substrate recognition. In addition to Fbxs, to which Fbxo7 belongs, there exist two other classes of F-box proteins, Fbws and Fbls. Fbxo7 recruits substrate via its C-terminal proline rich domain and associates with the adapter protein Skp1 via its F-box domain. In addition to mediating SCF-dependent ubiquitination, Fbxo7 is also involved in SCF-independent activities. Fbxo7 is reported to participate in autophagic clearance of damaged mitochondria (mitophagy) in response to mitochondrial depolarisation through direct interaction with PINK1 (PARK6) and Parkin (PARK2), while Parkinson's disease-associated Fbxo7 mutations affect Parkin mitochondrial translocation and Parkin-mediated mitophagy.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-Fbxo7 Antibody is an antibody against Fbxo7 for use in Western Blotting, Immunohistochemistry, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunohistochemistry Analysis: A 1:400 dilution from a representative lot detected Fbxo7 in human tonsil tissue.
      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Fbxo7 using U2OS cells transfected with constructs expressing either wildtype (T7-Fbxo7) or a deletion of F-box domain (T7-Fbxo7(ΔFbox)).
      Immunohistochemistry Analysis: A representative lot detected Fbxo7 in lung carcinoma and colon adenocarcinoma tissue (Laman, H., et al. (2005). EMBO. 24:3104-3116).
      Western Blotting Analysis: A representative lot detected Fbxo7 in U2OS cells, Asynchronous U2OS cells transfected with FBXO7, NIH3T3 cells transfected with Fbxo7(129–522) and FBXO7(deleted FBOX)) (Laman, H., et al. (2005). EMBO. 24:3104-3116).
      Western Blotting Analysis: A representative lot detected Fbxo7 in U2OS transfected with Fbxo7, U2OS (Kirk, R., et al. (2008). J. Biol. Chem. 283(32):22325-22335).
      Western Blotting Analysis: A representative lot detected Fbxo7 in Ba/F3 transfected with FBXO7,Ba/F3, Nalm-6, C-33 A, mouse spleen, liver, and cerebellum (Meziane, E.K., et al. (2011). J. Cell. Science. 124:2175-2186).
      Western Blotting Analysis: A representative lot detected Fbxo7 in U2OS transfected with FBXO7,Hek293, and SH-SY5Y (Burchell, V.S., et al. (2013). Nature Neuroscience. 16(9):1257-1265).
      Immunocytochemistry Analysis: A representative lot detected Fbxo7 in U2OS cells (Kirk, R., et al. (2008). J. Biol. Chem. 283(32):22325-22335).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human Fbxo7.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to cross react to all isoforms.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FBXO7
      • FBX7
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~65 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected Fbxo7 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1038 04055977169942