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ABC299 Anti-FFAR2 Antibody/GPR43

Anti-FFAR2/GPR43, Cat. No. ABC299, is a rabbit polyclonal antibody that detects FFAR2/GPR43 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-FFAR2 Antibody/GPR43 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: FFAR2, GPR43 UniProt Number: O15552
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ABC299
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC299
      DescriptionAnti-FFAR2 Antibody/GPR43
      Alternate Names
      • Free fatty acid receptor 2
      • G-protein coupled receptor 43
      • FFAR2/GPR43
      Background InformationFree fatty acid receptor 2 (UniProt: O15552; also known as G-protein coupled receptor 43) is encoded by the FFAR2 (also known as FFA2, GPCR43, GPR43) gene (Gene ID: 2867) in human. FFAR2 is a multi-pass membrane glycoprotein with four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. It is expressed at relatively high levels in peripheral blood leukocytes and at lower levels in the spleen. It is a G-protein-coupled receptor that is activated by short chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, SCFAs containing less than 6 carbons, such as acetate, propionate, and butyrate, produced by the gut microbiome, are the most potent activators. These SCFAs not only serve as a source of energy, but also act as signaling molecules. The activation of this G-protein-coupled receptor leads to the formation of inositol 1,4,5-trisphosphate leading to the mobilization of intracellular calcium and phosphorylation of ERK1 and ERK2. It also plays a role in glucose homeostasis by regulating the secretion of GLP-1. It is reported to regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel, FFAR2 also mediates the activation of the inflammatory chemokines and cytokines. FFAR2 is also involved in maintaining healthy gut microbiome and suppress intestinal carcinogenesis and its down modulation has been linked to colon cancers. (Ref.: Sivaprakasam, S., et al. (2016). Oncogenesis. 5; 238).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-FFAR2/GPR43, Cat. No. ABC299, is a rabbit polyclonal antibody that detects FFAR2/GPR43 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FFAR2/GPR43 in mouse kidney and mouse liver tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 54 amino acids from the C-terminal region of human Free fatty acid receptor 2 (FFAR2).
      EpitopeCytoplasmic domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Free fatty acid receptor 2 (FFAR2). It targets an epitope within the C-terminal region.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FFAR2
      • GPR43
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~50 kDa observed; 37.14 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected FFAR2/GPR43 in RAW264.7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC299 04053252985188

      Documentation

      Anti-FFAR2 Antibody/GPR43 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FFAR2 Antibody/GPR43 Certificates of Analysis

      TitleLot Number
      Anti-FFAR2 Antibody/GPR43 - 3694826 3694826
      Anti-FFAR2 Antibody/GPR43 - 3715715 3715715
      Anti-FFAR2 Antibody/GPR43 - 3739772 3739772
      Anti-FFAR2/GPR43 - Q2444408 Q2444408
      Anti-FFAR2/GPR43 - 3218239 3218239
      Anti-FFAR2/GPR43 - 3323154 3323154
      Anti-FFAR2/GPR43 - 3458523 3458523
      Anti-FFAR2/GPR43 - 3881001 3881001
      Anti-FFAR2/GPR43 - 4061134 4061134
      Anti-FFAR2/GPR43 - 4069778 4069778
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