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AB3284-200UL Anti-Endothelin Receptor B Antibody

Anti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB.

Anti-Endothelin Receptor B Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: CNS Control of Metabolism, Hormones & Receptors Gene Symbol: EDNRB, ET-B, ABCDS, HSCR2, ETRB, HSCR, ETB UniProt Number: P24530
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AB3284-200UL
200 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3284-200UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Endothelin Receptor B Antibody
      Alternate Names
      • ET-B
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationAnti-Endothelin Receptor B Antibody detects level of Endothelin Receptor B & has been published & validated for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200 using ECL on rat brain membranes.

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide corresponding to amino acids 298-314 of the rat Endothelin Receptor B (Accession P21451).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Endothelin Receptor B (ET-B). The immunogen sequence is specific for ET-B and is not present in any other known protein.

      SPECIES REACTIVITIES: The immunogen sequence is identical in all known vertebrate proteins. Reactivity with other species has not been confirmed.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Three transcript variants encoding two different isoforms have been found for this gene. While both isoforms bind ET1, they exhibit different responses upon binding, suggesting that they may be functionally distinct.
      Gene Symbol
      • EDNRB
      • ET-B
      • ABCDS
      • HSCR2
      • ETRB
      • HSCR
      • ETB
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24530 # Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
      SIZE: 442 amino acids; 49644 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.
      PTM: Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.
      DISEASE: SwissProt: P24530 # Defects in EDNRB are a cause of Waardenburg syndrome type IV (WS4) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4 is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). & Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). It is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child. & Defects in EDNRB are the cause of ABCD syndrome [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock at temporal occipital region, bilateral deafness, aganglionosis of the large intestine and total absence of neurocytes and nerve fibers in the small intestine.
      SIMILARITY: SwissProt: P24530 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB3284-200UL 04053252512346

      Documentation

      Anti-Endothelin Receptor B Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Endothelin Receptor B Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2420354 2420354
      RABBIT ANTI-ENDOTHELIN RECEPTOR B - 3269425 3269425
      RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY 2929215
      RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 1995583 1995583
      RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2281618 2281618

      Data Sheet

      Title
      RABBIT ANTI-ENDOTHELIN RECEPTOR B AFFINITY PURIFIED POLYCLONAL ANTIBODY