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ABE43 Anti-Ebp1 NT Antibody

Anti-Ebp1 N-terminal Antibody is a Rabbit Polyclonal Antibody for detection of Ebp1 also known as Proliferation-associated protein 2G4 & has been validated in WB, ICC.

Anti-Ebp1 NT Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins, Chromatin Biology Gene Symbol: EBP1, HG4-1, p38-2G4, PA2G4 UniProt Number: Q9UQ80
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ABE43
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE43
      Replaces07-397
      DescriptionAnti-Ebp1 NT Antibody
      Alternate Names
      • Proliferation-associated protein 2G4
      • Cell cycle protein p38-2G4 homolog
      • hG4-1
      • ErbB3-binding protein 1
      • Cell cycle protein p38-2G4 homolog
      • ErbB-3 binding protein 1
      • ErbB3-binding protein Ebp1
      Background InformationEbp1 is an RNA-binding protein involved in growth regulation. In cancer cells, this protein and has been implicated in growth inhibition and differentiation. Ebp1 is known to bind ribosomal and double-stranded RNA. It is a found in pre-ribosomal ribonucleoprotein complexes and is believed to play a role in ribosome assembly and rRNA processing. This protein is also a component of cytoplasmic bcl-2 messenger ribonucleoprotein. Through interactions with histion deacetylases, this protein can act transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes. Ebp1 also interacts with the cytoplasmic domain of the ErbB3 receptor, potentially transducing regulatory growth signals.
      References
      Product Information
      FormatSerum
      Control
      • A431 Cell Lysate.
      PresentationRabbit Polyclonal Serum with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ebp1 N-terminal Antibody is a Rabbit Polyclonal Antibody for detection of Ebp1 also known as Proliferation-associated protein 2G4 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry:
      A 1:500 dilution from a representative lot detected Ebp1 in NIH/3T3, A431, and HeLa cells.
      Biological Information
      ImmunogenLinear Peptide corresponding to the N-terminus of Ebp1.
      EpitopeN-terminus
      HostRabbit
      SpecificityRecognizes Ebp-1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq]
      Gene Symbol
      • EBP1
      • HG4-1
      • p38-2G4
      • PA2G4
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFunction: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site) By similarity. Ref.8 Ref.10 Ref.11 Ref.12



      Subunit structure: Interacts with the cytoplasmic domain of non-phosphorylated ERBB3; the interaction requires PKC activity. Interacts with AR. Treatment with HRG leads to dissociation from ERBB3 and increases association with AR. Interacts with NCL/nucleolin. Component of a ribonucleoprotein complex containing at least PA2G4, NCL, TOP1, PABPC2, RPLP0, acetylated histone H1 (HIST1H1A or H1F1), histone H1 2/4, RPL4, RPL8, RPL15, RPL18, RPL18A, RPL21, RPL11, RPL12, RPL28, RPL27, RPLP2 and RPL24. Interacts with HDAC2. Interacts with RB1; the interaction is enhanced upon PA2G4 dephosphorylation. Ref.8 Ref.10 Ref.12 Ref.2 Ref.7 Ref.19



      Subcellular location: Cytoplasm. Nucleus › nucleolus. Note: Tranlocates to the nucleus upon treatment with HRG. Ref.11 Ref.2 Ref.9



      Tissue specificity: Expressed in several cell lines tested, including primary and transformed cell lines. Ref.11



      Post-translational modification: Phosphorylated on serine and threonine residues. Phosphorylation is enhanced by HRG treatment. Basal phosphorylation is PKC-dependent and HRG-induced phosphorylation is predominantly PKC-independent. Ref.7 Ref.13 Ref.14 Ref.15 Ref.17 Ref.18



      Miscellaneous: Does not contain metal cofactors and does not have aminopeptidase activity.



      Sequence similarities: Belongs to the peptidase M24 family.
      Molecular Weight 48 kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE43 04053252676505

      Documentation

      Anti-Ebp1 NT Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ebp1 NT Antibody Certificates of Analysis

      TitleLot Number
      Anti-Ebp1 (N-terminus) - 2445967 2445967
      Anti-Ebp1 (N-terminus) - 2266794 2266794
      Anti-Ebp1 (N-terminus) - 3244011 3244011
      Anti-Ebp1 (N-terminus) - 3484038 3484038
      Anti-Ebp1 (N-terminus) - NG1946576 NG1946576
      Anti-Ebp1 (N-terminus) - NRG1702328 NRG1702328
      Anti-Ebp1 (N-terminus) -2746238 2746238
      Anti-Ebp1 (N-terminus) -2800514 2800514
      Anti-Ebp1 (N-terminus) Polyclonal Antibody 2967234
      Anti-Ebp1 (N-terminus) Polyclonal Antibody 2959550
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