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07-1820-25UL Anti-EEA1 (rabbit polyclonal)

This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.

Anti-EEA1 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: EEA1, ZFYVE2, MST105, MSTP105 UniProt Number: Q15075
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07-1820-25UL
25 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1820-25UL
      DescriptionAnti-EEA1 (rabbit polyclonal)
      Alternate Names
      • early endosome antigen 1
      • early endosome-associated protein
      • early endosome antigen 1, 162kD
      • Endosome-associated protein p162
      • Zinc finger FYVE domain-containing protein 2
      Background InformationEarly endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).
      References
      Product Information
      FormatAffinity Purified
      Control
      • BALB 3T3/A31 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.

      Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of human EEA1.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody recognizes EEA1.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human and Mouse.
      Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EEA1
      • ZFYVE2
      • MST105
      • MSTP105
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

      SUBUNIT STRUCTURE: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.

      SUBCELLULAR LOCATION: Cytoplasm. Early endosome membrane; Peripheral membrane protein.

      DOMAIN: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.

      MISCELLANEOUS: Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.

      SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger.

      Contains 1 FYVE-type zinc finger.
      Molecular Weight~ 170 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in BALB 3T3/A31 cell lysate.

      Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-1820-25UL 04054839350467