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AB10520 Anti-DAP-5 Antibody

This Anti-DAP-5 Antibody is validated for use in WB for the detection of DAP-5.

Anti-DAP-5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: EIF4G2, AAG1, DAP-5, DAP5, NAT1, p97 UniProt Number: P78344
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AB10520
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB10520
      DescriptionAnti-DAP-5 Antibody
      Alternate Names
      • Death-associated protein 5
      • aging-associated protein 1
      • eIF-4-gamma 2
      • eIF-4G 2
      • eIF4G 2
      • eukaryotic translation initiation factor 4 gamma, 2
      • eukaryotic translation initiation factor 4G-like 1
      Background InformationDeath Associated Protein 5 (DAP-5) is a protein with high amino acid sequence homology to Eukaryotic Translation Initiation Factor 4G (eIF4G). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferon-gamma induced cell death. DAP5 is cleaved at Asp790 during apoptosis . The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis.
      References
      Product Information
      FormatSerum
      Control
      • Jurkat cell lysate
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-DAP-5 Antibody is validated for use in WB for the detection of DAP-5.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human DAP-5.
      EpitopeUnknown
      HostRabbit
      SpecificityThis antibody recognizes DAP-5.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteProven to react with human and mouse. Predicted to react with rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTranslation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq]
      Gene Symbol
      • EIF4G2
      • AAG1
      • DAP-5
      • DAP5
      • NAT1
      • p97
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.

      SUBUNIT STRUCTURE: Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD.

      TISSUE SPECIFICTY: Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.

      PTM: Phosphorylation; hyperphosphorylated during mitosis.

      MISCELLANEOUS: This gene has been shown to be extensively edited in the liver of APOBEC1 transgenic animal model. Its aberrant editing could contribute to the potent oncogenesis induced by overexpression of APOBEC1. The aberrant edited sequence, called NAT1, is likely to be a fundamental translational repressor.

      SEQUENCE SIMILARITIES: Belongs to the eIF4G family.

      Contains 1 MI domain.

      Contains 1 MIF4G domain.

      Contains 1 W2 domain.
      Molecular Weight 97 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected DAP-5 on 10 µg of jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB10520 04053252732164