Anti-Cytomegalovirus Antibody, blend, clone 8B1.2, 1G5.2, and 2D4.2

Epitope: blend

MRC-5 infected cells with ATCC VR538 and AD169, ultrasonicated, screened by plate reduction neutralization tests.

Immunohistochemistry

Immunofluorescence

Optimal working dilutions must be determined by the end user.

This Anti-Cytomegalovirus Antibody, blend, clone 8B1.2, 1G5.2 & 2D4.2 is validated for use in IF, IH for the detection of Cytomegalovirus.

Reacts with Immediate early, early, and late antigen preparations. Can detect CMV infection within 2 hours post-infection exhibiting a nuclear staining which reaches peak intensity between 48 and 96 hours. This antigen is persisting and can be detected during the complete CMV infection cycle. Staining of CMV structural antigens becomes apparent at about 48 hours. Extra-nuclear staining is first seen in the region of the golgi apparatus followed subsequently by staining of viral proteins and particles in the cytoplasm.

With CMV the antigens expressed at different times are listed as:



Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD

0.02 M PB, 0.25M NaCl, PH 7.6 with 0.1% Sodium Azide.

Format: Purified

Maintain at +2-8C in convenient aliquots for up to 6 months. Do not freeze.

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