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ABE1974 Anti-CHMP1B

Anti-CHMP1B, Cat. No. ABE1974, is a highly specific rabbit polyclonal antibody that targets charged multivesicular body protein 1b and has been tested in Western Blotting.

Anti-CHMP1B MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CHMP1B, C18orf2 UniProt Number: Q7LBR1
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ABE1974
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1974
      DescriptionAnti-CHMP1B
      Alternate Names
      • Charged multivesicular body protein 1b
      • CHMP1.5
      • Chromatin-modifying protein 1b
      • Vacuolar protein sorting-associated protein 46-2
      • Vps46-2
      • hVps46-2
      Background InformationCharged multivesicular body protein 1b (UniProt: Q7LBR1; CHMP1.5, Chromatin-modifying protein 1b, CHMP1b, Vacuolar protein sorting-associated protein 46-2, Vps46-2, hVps46-2) is encoded by the CHMP1B (also known as C18orf2) gene (Gene ID: 57132) in human. CHMP1b is a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. It is widely expressed and is found in pancreas, kidney, skeletal muscle, liver, lung, placenta and the brain tissue. CHMP1b is a peripherally associated component of the endosomal sorting system that is required for transport complex III (ESCRT-III), which is involved in multi-vesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. CHMP1b is localized in cytosol, endosome, and in peripheral membrane protein. CHMP1b binds specifically to the AAA ATPase spastin and thereby recruits this microtubule severing protein to the midbody. CHMP1b and spastin are shown to colocalize at midbodies in dividing HeLa cells, and the knockdown of CHMP1b reduces the amount of spastin at midbodies.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CHMP1B, Cat. No. ABE1974, is a highly specific rabbit polyclonal antibody that targets charged multivesicular body protein 1b and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected CHMP1B in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad. Sci USA. 107(29):12889-94; Bailey, J.K., et. al. (2015). J Biol Chem. 290(14):8987-9001).
      Biological Information
      ImmunogenGST-tagged full length recombinant human Charged multivesicular body protein 1b.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects Charged multivesicular body protein 1b in human cells. Human CHMP1B displays 99% and 98% homology with bovine and mouse CHMP1B, respectively.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react bovine and mouse based on 99 and 98% homology, respectively.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHMP1B
      • C18orf2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~29 kDa observed; 22.11 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in ARPE-19 cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected CHMP1B in 10 µg of ARPE-19 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1974 04054839201004