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ABS1616 Anti-CGI-58 Antibody

Detect CGI-58 using this Anti-CGI-58 Antibody validated for use in Western Blotting and Immunocytochemistry.

Anti-CGI-58 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: Abhd5 UniProt Number: Q9DBL9
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ABS1616
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1616
      DescriptionAnti-CGI-58 Antibody
      Alternate Names
      • 1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (EC:2.3.1.51)
      • Abhydrolase domain-containing protein 5
      • Lipid droplet-binding protein CGI-58
      • Protein CGI-58
      • CGI-58
      Background Information1-acylglycerol-3-phosphate O-acyltransferase ABHD5 (EC:2.3.1.51; UniProt Q9DBL9; also known as Abhydrolase domain-containing protein 5, CGI-58, Comparative gene identification-58, Lipid droplet-binding protein CGI-58) is encoded by the Abhd5 (also known as CDS, IECN5, NCIE2, 1300003D03Rik, 2010002J10Rik) gene in murine species (Gene ID 67469). The comparative gene identification-58 (CGI-58) functions as a cofactor of adipose triglyceride lipase/ATGL-mediated triacylglycerol (TG) hydrolytic activity, which is essential for efficient catabolism of cellular TG stores. Mutations in the genes coding for ATGL or CGI-58 in humans cause neutral lipid storage disease characterized by TG accumulation in multiple tissues. CGI-58 gene mutations provoke severe ichthyosis and hepatosteatosis in humans and mice. Mice lacking CGI-58 exclusively in muscle (CGI-58KOM) show TG accumulation in both skeletal and cardiac muscle tissues and develop severe cardiac steatosis and cardiomyopathy as a result of impaired TG catabolism and mitochondrial fatty acid oxidation. Immunocytochemistry analysis reveals diffuse cytoplasmic staining of CGI-58 in 3T3-L1 preadipocytes, while CGI-58 staining is found exclusively on the surface of lipid droplets in differentiated 3T3-L1 adipocytes. CGI-58 binds to lipid droplets by interacting with perilipin C-terminal sequence, while activation of cellular PKA disperses CGI-58 localization from the lipid droplets to cytosol.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CGI-58 using this Anti-CGI-58 Antibody validated for use in Western Blotting and Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:5,000 dilution from a representative lot detected CGI-58 in 3T3-L1 adipocytes (Data courtesy of Dawn Brasaemle, Rutgers University).
      Western Blotting Analysis: A representative lot detected recombinant CGI-58, as well as endogenous CGI-58 in differentiated 3T3-L1 adipocytes, and 3T3-L1 preadipocytes stably expressing ectopic perilipin A or perilipin B (Subramanian, V., et al. (2004). J. Biol. Chem. 279(40):42062-42071).
      Immunocytochemistry Analysis: A representative lot detected CGI-58 in 3T3-L1 adipocytes, 3T3-L1 preadipocytes stably expressing perilipin A (Subramanian, V., et al. (2004). J. Biol. Chem. 279:42062-42071).
      Western Blotting Analysis: A representative lot detected CGI-58 in mouse cardiac muscle, liver, white and brown adipose tissue (Zierler, K.A., et al. (2013). J. Biol. Chem. 288(14):9892-9904).
      Western Blotting Analysis: A representative lot detected CGI-58 in in mouse OP9 adipocytes and brown adipose tissue (Skinner, J.R., et al. (2013). Adipocyte. 2(2):80-86).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to mouse CGI-58.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Abhd5
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~42 kDa observed. CGI-58 migrates with an apparent molecular size slightly larger than the calculated molecular weight of 39.2 kDa for mouse CGI-58.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in differentiated 3T3-L1 adipocytes cell lysate.

      Western Blotting Analysis: A 1:1,000 of this antibody detected CGI-58 in 10 µg of differentiated 3T3-L1 adipocytes cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1616 04055977173024