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MAB4399-I Anti-CD133 (Prominin-1) Antibody, clone 17A6.1

Detect CD133 using this mouse monoclonal Anti-CD133 (Prominin-1) antibody, clone 17A6.1, Cat. No. MAB4399-I. Validated for use in Immunocytochemistry.

Anti-CD133 (Prominin-1) Antibody, clone 17A6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: PROM1, PROML1, MSTP061 UniProt Number: O43490
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MAB4399-I
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB4399-I
      DescriptionAnti-CD133 (Prominin-1) Antibody, clone 17A6.1
      Alternate Names
      • Prominin-1
      • Prominin-like protein 1
      • Antigen CD133
      Background InformationProminin-1 (UniProt: O43490; also known as Antigen AC133, Prominin-like protein 1, CD133) is encoded by the PROM1 (also known as PROML1, MSTP061) gene (Gene ID: 8842) in human. CD133 (Prominin-1) is multi-pass cell-surface glycoprotein that plays a role in cell differentiation, proliferation and apoptosis. It binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development CD133 acts as a key regulator of disk morphogenesis. Defects in PROM1 gene are known to cause retinitis pigmentosa, cone-rod dystrophy, and retinal macular dystrophy 2. Higher levels of CD133 have been reported in several cancer cell types, including various leukemias, retinoblastomas, glioblastomas, and kidney carcinomas. Higher levels of CD133 are also observed in pancreatic, gastric, colorectal, and hepatocellular cancers. It is also considered as a marker of stem cells in the adult small intestine that are susceptible to transformation into tumors retaining a fraction of mutant Prom1+ tumor cells. (Ref.: Zhu, L., et al. (2009). Nature 477 (7229); 603-607).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CD133 using this mouse monoclonal Anti-CD133 (Prominin-1) antibody, clone 17A6.1, Cat. No. MAB4399-I. Validated for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected CD133 in U251 cells.
      Biological Information
      ImmunogenGST-tagged recombinat fragment corresponding to 77 amino acids from the N-terminal region of human CD133 (Prominin-1).
      Clone17A6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 17A6.1 detects recombinant CD133 (Prominin-1) by Western blotting and also detects CD133 (Prominin-1 ) in U251 cells by immunocytochemistry.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PROM1
      • PROML1
      • MSTP061
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~97 kDa observed; 97.2 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant Prominin-1.

      Western Blotting Analysis: 1 µg/mL of this antibody detected 2.5 µg of recombinant Prominin-1 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB4399-I 04054839155987

      Documentation

      Anti-CD133 (Prominin-1) Antibody, clone 17A6.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CD133 (Prominin-1) Antibody, clone 17A6.1 Certificates of Analysis

      TitleLot Number
      Anti-CD133 (Prominin-1), 3050612
      Anti-CD133 (Prominin-1), -Q2805306 Q2805306
      Anti-CD133 (Prominin-1), clone 17A6.1 - 3244390 3244390
      Anti-CD133 (Prominin-1), clone 17A6.1 - 3776715 3776715
      Anti-CD133 (Prominin-1), clone 17A6.1 - 3994673 3994673
      Anti-CD133 (Prominin-1), clone 17A6.1 - 4097739 4097739
      Anti-CD133 (Prominin-1), clone 17A6.1 -2892457 2892457
      Anti-CD133 (Prominin-1), clone 17A6.1 Monoclonal Antibody 3158274
      Anti-CD133 (Prominin-1), clone 17A6.1 Monoclonal Antibody 2920698