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MABE89 Anti-Brm Antibody, clone 1H7A10

Use Anti-Brm Antibody, clone 1H7A10 (Rat Monoclonal Antibody) validated in WB, IHC to detect Brm also known as ATP-dependent helicase SMARCA2 or SNF2/SWI2-like protein 2.

Anti-Brm Antibody, clone 1H7A10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: BAF190B, BRM, hBRM, SMARCA2, SNF2L2, Sth1p, SWI2, SNF2, SNF2-like 2, BAF190, SNF2LA, hSNF2a, SNF2A, SNF2-alpha UniProt Number: Q6DIC0
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MABE89
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IHCRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE89
      DescriptionAnti-Brm Antibody, clone 1H7A10
      Alternate Names
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
      • ATP-dependent helicase SMARCA2
      • probable global transcription activator SNF2L2
      • sucrose nonfermenting 2-like protein 2
      • SNF2/SWI2-like protein 2
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2
      • global transcription activator homologous sequence
      • Protein brahma homolog
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
      • BRG1-associated factor 190B
      Background InformationBAF190B (BRG1-associated factor 190B), also known as protein brahma homolog (Brm), and SNF2-alpha, functions as a transcriptional coactivator. This protein contains a helicase domain and is a member of the SWI/SNF family of proteins and is part of the SWI-SNF chromatin remodeling complex. The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. This large complex contains multiple proteins. BAF190 (Brm) and Brg-1 (SNF2-beta) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, or SNF5), BAF155 (SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
      References
      Product Information
      FormatPurified
      Control
      • K562 nuclear extract
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-Brm Antibody, clone 1H7A10 (Rat Monoclonal Antibody) validated in WB, IHC to detect Brm also known as ATP-dependent helicase SMARCA2 or SNF2/SWI2-like protein 2.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunocytochemistry:
      A representative lot of this antibody was reported to work in IC using C2C12 cells (Yasuyuki Ohkawa, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.)

      Immunohistochemistry:
      A representative lot of this antibody was reported to work in IHC using mouse embryonic and postnatal brain (Yasuyuki Ohkawa, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.)
      Biological Information
      ImmunogenRecombinant protein corresponding to amino acids 48-214 of mouse Brm.
      Clone1H7A10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      Specificity Recognizes BAF190B also known as Brm
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BAF190B
      • BRM
      • hBRM
      • SMARCA2
      • SNF2L2
      • Sth1p
      • SWI2
      • SNF2
      • SNF2-like 2
      • BAF190
      • SNF2LA
      • hSNF2a
      • SNF2A
      • SNF2-alpha
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Ref.2

      SUBUNIT STRUCTURE: Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Binds TOPBP1 (By similarity). Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Interacts with PHF10/BAF45A. Ref.2

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.

      Contains 1 bromo domain.

      Contains 1 helicase ATP-binding domain.

      Contains 1 helicase C-terminal domain.

      Contains 1 HSA domain.
      Molecular Weight ~140-180 kDa observed. Uncharacterized lower molecular weight bands have been reported in some lysates and may represent splice variants and/or degradation products. See Batsché E, Yaniv M, Muchardt C. Nat Struct Mol Biol. 2006 Jan;13(1):22-29.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 nuclear extract.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Brm on 10 µg of K562 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE89 04053252482496

      Documentation

      Anti-Brm Antibody, clone 1H7A10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Brm Antibody, clone 1H7A10 Certificates of Analysis

      TitleLot Number
      Anti-Brm, clone 1H7A10 - 2345043 2345043
      Anti-Brm, clone 1H7A10 - 2439675 2439675
      Anti-Brm, clone 1H7A10 - 3208172 3208172
      Anti-Brm, clone 1H7A10 - 3674952 3674952
      Anti-Brm, clone 1H7A10 - 3874305 3874305
      Anti-Brm, clone 1H7A10 - 3891817 3891817
      Anti-Brm, clone 1H7A10 - 4019032 4019032
      Anti-Brm, clone 1H7A10 - NRG1843061 NRG1843061
      Anti-Brm, clone 1H7A10 -2492150 2492150
      Anti-Brm, clone 1H7A10 -2591905 2591905
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