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MABT1350-25UG Anti-Autotaxin (ENPP2) Antibody, clone 7A5

Anti-Autotaxin (ENPP2), clone 7A5, Cat. No. MABT1350, is a mouse monoclonal antibody that detects Autotaxin (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2) and has been tested for u

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Anti-Autotaxin (ENPP2) Antibody, clone 7A5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: ENPP2, ATX, PDNP2 UniProt Number: Q13822
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MABT1350-25UG
25 μg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HICC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1350-25UG
      DescriptionAnti-Autotaxin (ENPP2) Antibody, clone 7A5
      Alternate Names
      • Ectonucleotide pyrophosphatase/phosphodiesterase family member 2
      • EC: 3.1.4.39
      • E-NPP 2
      • Extracellular lysophospholipase D
      • LysoPLD
      Background InformationEctonucleotide pyrophosphatase/phosphodiesterase family member 2 (UniProt: Q13822; also known as EC: 3.1.4.39, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD) is encoded by the ENPP2 (also known as ATX, PDNP2) gene (Gene ID: 5168) in human. Autotaxin is a secreted glycoprotein that is present in biological fluids. Its lysophospholipase D catalyzes the production of lysophosphatidic acid (LPA) in extracellular fluids. The level of LPA is shown to be markedly increased in the bronchoalveolar lavage fluid in asthma patients in response to allergens. Lysophosphatidylcholine is shown to be its major substrate. It can also act on sphingosylphosphphorylcholine to produce sphingosine-1-phosphate, a modulator of cell motility. Autotaxin is synthesized with a signal peptide (aa 1-27) and a propeptide (aa 28-35), which is cleaved off by furin in the mature form. Three isoforms of Autotaxin have been reported that are produced by alternative splicing. Isoform 1 is highly expressed in peripheral tissues and isoform 3 is more prominently expressed in the brain. Adipocytes are shown to express only isoform 1. Autotaxin levels are up-regulated in obese subjects with glucose intolerance and during adipogenesis. Autotaxin can also act as an angiogenic factor that stimulates migration of smooth muscle cells and microtubule formation and increased expression of Autotaxin has been reported in various types of cancers. (Ref.: Park, GY., et al. (2013). Am. J. Respir. Crit. Care Med. 188(8); 928 940; Oikonomou, N., et al. (2012). Am. J. Respir. Cell Mol. Biol. 47(5); 566-574).
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Autotaxin (ENPP2), clone 7A5, Cat. No. MABT1350, is a mouse monoclonal antibody that detects Autotaxin (Ectonucleotide pyrophosphatase/phosphodiesterase family member 2) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Autotaxin (ENPP2) in human kidney and human pancreas tissue sections.

      Immunohistochemistry Analysis: A representative lot detected Autotaxin (ENPP2) in Immunohistochemistry applications (Kaffe, E., et. al. (2017). Hepatology. 65(4):1369-1383).

      Immunocytochemistry Analysis: A representative lot detected Autotaxin (ENPP2) in Immunocytochemistry applications (Kaffe, E., et. al. (2017). Hepatology. 65(4):1369-1383).

      Western Blotting Analysis: A representative lot detected Autotaxin (ENPP2) in Western Blotting applications (Kaffe, E., et. al. (2017). Hepatology. 65(4):1369-1383).
      Biological Information
      ImmunogenHis-tagged recombinant fragment (without the first 28 amino acids) from human Autotaxin.
      Clone7A5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7A5 detects human and murine autotaxin.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ENPP2
      • ATX
      • PDNP2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~100 kDa observed; 98.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human autotaxin.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected recombinant human autotaxin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1350-25UG 04054839540196